Strain or plasmid | Description | Source |
---|---|---|
Escherichia coli | ||
DH5α | supE44ΔlacU169(Φ80lacZΔM15) hsdR17recA1 gyrA96thi-1relA1 | [20] |
BL21(DE3) | hsdS gal(λcIts857 ind1 Sam7 nin5 lacUV5-T7 gene 1) | Novagen |
Pectobacterium carotovorum subsp. carotovorum | ||
H-rif-8-6 | Pcc, Rifr, wild-type | Laboratory stock |
TH22–10 | H-rif-8-6, CaroK1::Tn5, Rifr, Kanr | This study |
Ea1068 | Pcc, wild-type | Laboratory stock |
SP33 | Pcc, wild-type | Laboratory stock |
Plasmid | ||
pGEM T-Easy | Ampr; lacZ cloning vector | Promega |
pEN3KI | caroS3K subcloned into PET32a | This study |
pET32a | Ampr; expression vector with the N-terminal His-tag | Novagen |
pES3KI | Derived from pEN3K; deleted series of Tag element in front of the expressed caroS3K | This study |
pES3I | Derived from pECS3I, the (His)6-Tag element was deleted | This study |