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Table 1 Bacteria and plasmids used in the study

From: Identification and characterization of the bacteriocin Carocin S3 from the multiple bacteriocin producing strain of Pectobacterium carotovorum subsp. carotovorum

Strain or plasmid

Description

Source

Escherichia coli

 DH5α

supE44ΔlacU169(Φ80lacZΔM15) hsdR17recA1 gyrA96thi-1relA1

[20]

 BL21(DE3)

hsdS gal(λcIts857 ind1 Sam7 nin5 lacUV5-T7 gene 1)

Novagen

Pectobacterium carotovorum subsp. carotovorum

 H-rif-8-6

Pcc, Rifr, wild-type

Laboratory stock

 TH22–10

H-rif-8-6, CaroK1::Tn5, Rifr, Kanr

This study

 Ea1068

Pcc, wild-type

Laboratory stock

 SP33

Pcc, wild-type

Laboratory stock

Plasmid

 pGEM T-Easy

Ampr; lacZ cloning vector

Promega

 pEN3KI

caroS3K subcloned into PET32a

This study

 pET32a

Ampr; expression vector with the N-terminal His-tag

Novagen

 pES3KI

Derived from pEN3K; deleted series of Tag element in front of the expressed caroS3K

This study

 pES3I

Derived from pECS3I, the (His)6-Tag element was deleted

This study

  1. Kanr Kanamycin, Cmlr Chloramphenicol, Rifr Rifampicin, Ampr Ampicillin