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Table 1 Bacteria and plasmids used in the study

From: Identification and characterization of the bacteriocin Carocin S3 from the multiple bacteriocin producing strain of Pectobacterium carotovorum subsp. carotovorum

Strain or plasmid Description Source
Escherichia coli
 DH5α supE44ΔlacU169(Φ80lacZΔM15) hsdR17recA1 gyrA96thi-1relA1 [20]
 BL21(DE3) hsdS gal(λcIts857 ind1 Sam7 nin5 lacUV5-T7 gene 1) Novagen
Pectobacterium carotovorum subsp. carotovorum
 H-rif-8-6 Pcc, Rifr, wild-type Laboratory stock
 TH22–10 H-rif-8-6, CaroK1::Tn5, Rifr, Kanr This study
 Ea1068 Pcc, wild-type Laboratory stock
 SP33 Pcc, wild-type Laboratory stock
Plasmid
 pGEM T-Easy Ampr; lacZ cloning vector Promega
 pEN3KI caroS3K subcloned into PET32a This study
 pET32a Ampr; expression vector with the N-terminal His-tag Novagen
 pES3KI Derived from pEN3K; deleted series of Tag element in front of the expressed caroS3K This study
 pES3I Derived from pECS3I, the (His)6-Tag element was deleted This study
  1. Kanr Kanamycin, Cmlr Chloramphenicol, Rifr Rifampicin, Ampr Ampicillin