Establishment and application of a multiple cross displacement amplification combined with nanoparticles-based biosensor method for the detection of Bordetella pertussis

Table 1 The primers used in this study

Primers name ^a	Sequences and modifications ^b	Length ^c	Gene
F1	5′- CGCATACGTGTTGGCAAC-3’	18 nt	PT promoter region
F2	5′-GCGTTTTGATGGTGCCT-3’	17 nt
CP1	5′-GATTGCAGTAGCGGGATGTACCATGCGTGCAGATTCGT −3’	38 mer
CP2	5′-GCGATGGTACCGGTCACCGTACGGATCACACCATGGC-3’	37 mer
C1	5′-GATTGCAGTAGCGGGATGTAC-3’	21 nt
C1*	5′-Biotin-GATTGCAGTAGCGGGATGTAC-3’	21 nt
C2	5′-GCGATGGTACCGGTCACCGT-3’	20 nt
D1	5′-GAAGAATCGAGGGTTTTGTAC-3’	21 nt
D2	5′-CCGGACCGTGCTGACC-3’	16 nt
R1	5′-GAGCGTTCATGCCGTGT-3’	17 nt
R1*	5′-FITC-GAGCGTTCATGCCGTGT-3’	17 nt
R2	5′-CTTCGGCGCAAAGTCGC-3’	17 nt

^a C1*, 5′-labeled with biotin when used in MCDA-LFB assay; R1*, 5′-labeled with FITC when used in MCDA-LFB assay. ^b FITC fluorescein isothiocyanate, ^c mer monomeric unit, nt nucleotide, PT pertussis toxin

ISSN: 1471-2180