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Table 1 The primers used in this study

From: Establishment and application of a multiple cross displacement amplification combined with nanoparticles-based biosensor method for the detection of Bordetella pertussis

Primers name a Sequences and modifications b Length c Gene
F1 5′- CGCATACGTGTTGGCAAC-3’ 18 nt PT promoter region
F2 5′-GCGTTTTGATGGTGCCT-3’ 17 nt
CP1 5′-GATTGCAGTAGCGGGATGTACCATGCGTGCAGATTCGT −3’ 38 mer
CP2 5′-GCGATGGTACCGGTCACCGTACGGATCACACCATGGC-3’ 37 mer
C1 5′-GATTGCAGTAGCGGGATGTAC-3’ 21 nt
C1* 5′-Biotin-GATTGCAGTAGCGGGATGTAC-3’ 21 nt
C2 5′-GCGATGGTACCGGTCACCGT-3’ 20 nt
D1 5′-GAAGAATCGAGGGTTTTGTAC-3’ 21 nt
D2 5′-CCGGACCGTGCTGACC-3’ 16 nt
R1 5′-GAGCGTTCATGCCGTGT-3’ 17 nt
R1* 5′-FITC-GAGCGTTCATGCCGTGT-3’ 17 nt
R2 5′-CTTCGGCGCAAAGTCGC-3’ 17 nt
  1. a C1*, 5′-labeled with biotin when used in MCDA-LFB assay; R1*, 5′-labeled with FITC when used in MCDA-LFB assay. b FITC fluorescein isothiocyanate, c mer monomeric unit, nt nucleotide, PT pertussis toxin