Table 4 Oligonucleotide primers used to detect ESBL genes in E. coli isolates
| Genes | Primer sequences | Expected amplicon size (bp) |
|---|---|---|
| TEM-1 |
Forward: 5′-GAGACAATAACCCTGGTAAAT-3′ Reverse: 5′-AGAAGTAAGTTGGCAGCAGTG-3′ | 459 |
| CTX-M |
Forward: 5′-GAAGGTCATCAAGAAGGTGCG-3′ Reverse: 5′-GCATTGCCACGCTTTTCATAG-3′ | 560 |
| SHV |
Forward: 5′-GTCAGCGAAAAACACCTTGCC’ Reverse: 5′-GTCTTATCGGCGATAAACCAG-3’ | 383 |