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Table 4 Oligonucleotide primers used to detect ESBL genes in E. coli isolates

From: Antimicrobial resistance and molecular detection of extended spectrum β-lactamase producing Escherichia coli isolates from raw meat in Greater Accra region, Ghana

Genes Primer sequences Expected amplicon size (bp)
TEM-1 Forward: 5′-GAGACAATAACCCTGGTAAAT-3′
Reverse: 5′-AGAAGTAAGTTGGCAGCAGTG-3′
459
CTX-M Forward: 5′-GAAGGTCATCAAGAAGGTGCG-3′
Reverse: 5′-GCATTGCCACGCTTTTCATAG-3′
560
SHV Forward: 5′-GTCAGCGAAAAACACCTTGCC’
Reverse: 5′-GTCTTATCGGCGATAAACCAG-3’
383