Table 4 Oligonucleotide primers used to detect ESBL genes in E. coli isolates
Genes | Primer sequences | Expected amplicon size (bp) |
|---|---|---|
TEM-1 | Forward: 5′-GAGACAATAACCCTGGTAAAT-3′ Reverse: 5′-AGAAGTAAGTTGGCAGCAGTG-3′ | 459 |
CTX-M | Forward: 5′-GAAGGTCATCAAGAAGGTGCG-3′ Reverse: 5′-GCATTGCCACGCTTTTCATAG-3′ | 560 |
SHV | Forward: 5′-GTCAGCGAAAAACACCTTGCC’ Reverse: 5′-GTCTTATCGGCGATAAACCAG-3’ | 383 |