Fig. 4

Pseudonajide is located on the cell envelope and inside the bacterial cell. a Confocal microscopy images of Staphylococcus epidermidis incubated for 1, 4, and 24 h with 25 μM pseudonajide tagged with fluorescein isothiocyanate (FITC). Scale, 10 μm. Cells were washed once with saline solution, and 3 μL cell suspension were added to each glass slide. b Graph demonstrating the percentage of cells that were fluorescent at each time point. Shown are 5 random fields, with approximately 1000 cells counted for each time point. Calculated by dividing the number of fluorescent cells by the total number of cells in the field. Images were analyzed using Fiji software