Fig. 3
Antimicrobial activity of pseudonajide (peptide 2). Staphylococcus epidermidis was cultured in the same conditions as the biofilm tests, but with a different pseudonajide concentration (25 μM). a Optical density measurements at 600 nm (OD600) show a reduction in bacterial growth in the presence of 25 μM pseudonajide (peptide 2) after 1, 2, and 4 h. b Colony-forming unit (CFU) testing confirmed the reduction in cell viability as compared to the control. All the tests were performed with at least 3 different biological replicates, each having at least 3 technical replicates. Error bars are shown, and statistical analysis was performed applying Student’s t-test, where: *, p < 0.05; **, p < 0.005; NS, non-significant