Fig. 12

Dam independently promoted NLRP3 inflammasome activation. J774A.1 cells were transduced with LV5-Dam or LV5-negative lentivirus, untreated cells was used as a negative control (Blank). Cells were then pre-treated with LPS (1 μg/mL, 5 h) and stimulated with or without ATP (1.25 mM) for 1 h. The production of IL-1β a and IL-6 b in the supernatants were examined via ELISA. ***p < 0.001 for one-way ANOVA followed by Bonferroni’s multiple comparison test indicate significant findings in comparison with the control group. Data are presented as mean ± SEM of triplicate samples per experimental condition from three independent experiments. c. The expression of caspase-1, NLRP3, ASC, pro-IL-1β, and P-Jnk were analyzed by immunoblotting. β-actin was blotted as a loading control. Molecular mass markers in kDa are indicated on the right. Original images of immunoblotting were shown in Fig. S8