Fig. 6

Wogonin inhibited HSV-2-induced JNK and p38 MAPK activation. a to c Wogonin inhibited HSV-2-induced JNK and p38 MAPK phosphorylation. HEC-1-A cells were mock-infected or infected with HSV-2 (MOI = 1) in the presence or absence of wogonin (100 μM). The levels of JNK, p38 MAPK, their phosphorylated forms and the downstream factors p-c-Jun andp-ATF-2 were determined by western blot 24 h p.i. d Wogonin inhibited HSV-2-induced AP-1 activation. HEC-1-A cells were transfected with an AP-1-luc reporter plasmid and then the cells were mock-treated or treated with serial concentrations of wogonin, SB203580 (20 μM) or SP600125 (20 μM) prior to mock infection or infection with HSV-2 (MOI = 1). SB203580 and SP600125, which are p38/MAPK and JNK inhibitors, respectively, were used as the positive controls. The relative luciferase activity was determined after 24 h. The data are presented as mean values (±SDs) of triplicate determinations from three independent experiments