Fig. 2

Wogonin inhibited HSV L gene expression. a and b Wogonin inhibited HSV-1/2 gD mRNA transcription in HEC-1-A cells. Cells were pretreated with serial concentrations of wogonin for 30 min, and then infected with HSV-1 (HF) or HSV-2 (G) (MOI = 1). HSV-1/2 gD mRNA transcript levels were determined via qPCR 24 h p.i. c and d Wogonin suppressed HSV-1/2 gD protein expression in HEC-1-A cells c and Vero cells d. Cells were treated with wogonin prior to infection with HSV-1 or HSV-2 (MOI = 1). The gD protein expression level was determined via western blot 24 h p.i. e The cytotoxicity of wogonin was investigated by CCK-8 colorimetric assay after 48 h of compound exposure. All experiments were performed three times. Representative results are shown. The data are the mean values (±SDs) of triplicate determinations