Fig. 4
Effect of peptide BP178 on viability and culturability of Xff strain Temecula at different peptide concentrations. Total cell concentration was estimated by conventional qPCR (white symbols), cell viability was estimated by v-qPCR (black symbols), and cell culturability by plate counting (grey symbols). Exposure times of 3 h (triangles) and 24 h (circles) were used. Cell concentration was 1 × 107 CFU/ml in both cases. The dash line represents the detection limit of v-qPCR, whereas the normal line indicates the detection limit of the plate counting technic. Values are the means of three replicates, and error bars represent the standard deviation of the mean. Letters correspond to the means comparison of viable cells treated with BP178 at exposure time of 3 h. Means sharing the same letters are not significantly different (P < 0.05), according to the Tukey’s test