Fig. 3
Effect of peptide BP178 on viability and culturability of Xff strain Temecula at different exposure times. Cell viability was estimated by v-qPCR (black symbols) and cell culturability by plate counting (grey symbols). Initial cell concentration was 1 × 107 CFU/ml and the BP178 concentration used was 50 μM. Non-treated controls (NTC) were also performed by adding the corresponding volume of sterile distilled water. The dash line represents the detection limit of v-qPCR, whereas the normal line indicates the detection limit of the plate counting technic. Values are the means of three replicates, and error bars represent the standard deviation of the mean. Lowercase letters correspond to the means comparison of viable cells treated with BP178 (black triangles). Capital letters correspond to the means comparison of culturable cells treated with BP178 (grey triangles). Means sharing the same letters are not significantly different (P < 0.05), according to the Tukey’s test