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Construction of deletion mutants
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|
0046HindIIIF
|
GATCTAAAGCTTGCTCAACATTATTAACGGTTC
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|
0046R
|
TTCTAACATAATGAATACCTCGTA
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|
0046Soe
|
AGGTATTCATTATGTTAGAAGACTAATCTAGATCAAGATTCATCA
|
|
0046SacIR
|
GATCTAGAGCTCCATACTACTTCTGCGTCTTC
|
|
0864HindIIIF
|
GATCTAAAGCTTCTGCTGATATTGATGCAGTGAGTGG
|
|
0864R
|
CGCACCGGCAATAACTATTCCCTTAAT
|
|
0864Soe
|
AAGGGAATAGTTATTGCCGGTGCGCGTGCAGAATTAACTCAAGGTCGC
|
|
0864SacIR
|
GATCTAGAGCTCCGTGCACTTGACACAGATCCTG
|
|
1426HindIIIF
|
GATCTAAAGCTTTATAGATTAATTGACTGCAGCC
|
|
1426R
|
CGCTGCCATTGAAGTAATTA
|
|
1426Soe
|
TAATTACTTCAATGGCAGCGAACTAATCTATTAATGAAGAAACTCGT
|
|
1426SacIR
|
GATCTAGAGCTCCTTATCGTTCATGCCAAGAA
|
|
1539BamHIF
|
GATCTAGGATCCATCACTTGATCGATCATCTG
|
|
1539R
|
CTTCATCTGAATATCTCCTCT
|
|
1539Soe
|
AGAGGAGATATTCAGATGAAGTTGATAAAATAATCTACTACTTTGTGA
|
|
1539SacIR
|
GATCTAGAGCTCCTCTTAGGTGCAAGCATTAA
|
|
PCR analysis and DNA sequencing of deletion targets
|
|
0046up
|
CTATCTGTATGATGCTTCCAC
|
|
0046dw
|
GTACCTCAATCTGTTGTAATCTC
|
|
0864up
|
ACAAGCTAGAGGTATGGCTGG
|
|
0864dw
|
CCACATGAATGGCGTATGGC
|
|
1426up
|
AAGCCGTTGTATTGAATGATGGTAG
|
|
1426dw
|
CGCGAATCATCAATTCACGGTA
|
|
1539up
|
CAGGATAGGGATGCACATGC
|
|
1539dw
|
CGACGTTGACGTGTTACTGT
|
