|
Strains
|
|
Lactobacillus acidophilus
|
|
NCK56 (NCFM)
|
Human intestinal isolate
|
[28]
|
|
NCK1909 (Δupp)
|
NCFM with a 315 bp in-frame deletion within the upp gene (lba0770); background/parent strain for NCFM deletion mutants
|
[27]
|
|
NCK1910
|
NCK1909 harboring the repA helper plasmid pTRK669; host for pORI-based counterselective integration vector
|
[27]
|
|
NCK2439 (Δlba0864)
|
NCK1909 with a 1425 bp in-frame deletion within the lba0864 gene
|
This study
|
|
NCK2441 (Δlba1426)
|
NCK1909 with a 717 bp in-frame deletion within the lba1426 gene
|
This study
|
|
NCK2530 (Δlba0046)
|
NCK1909 with a 342 bp in-frame deletion within the lba0046 gene
|
This study
|
|
NCK2608 (Δlba1539)
|
NCK1909 with a 498 bp in-frame deletion within the lba1539gene
|
This study
|
|
Escherichia coli
|
|
EC101
|
RepA+ JM101; Kmr; repA from pWV01 integrated in chromosome; cloning host for pORI-based plasmids
|
[29]
|
|
NCK1391
|
E. coli DH10B harboring pTRK669; cloning host for pORI-based plasmids
|
[27]
|
|
NCK1911
|
EC101 host harboring pTRK935 integration vector
|
[27]
|
|
NCK2438
|
EC101 host harboring pTRK1118 recombinant plasmid
|
This study
|
|
NCK2440
|
EC101 host harboring pTRK1119 recombinant plasmid
|
This study
|
|
NCK2529
|
EC101 host harboring pTRK1126 recombinant plasmid
|
This study
|
|
NCK2607
|
DH10B host harboring pTRK1170 recombinant plasmid
|
This study
|
|
Plasmids
|
|
pTRK669
|
Ori (pWV01), Cmr, RepA+, thermosensitive
|
[30]
|
|
pTRK935
|
pORI upp-based counterselective integration vector, Emr
|
[27]
|
|
pTRK1118
|
pTRK935 harboring a mutated copy of lba0864 gene cloned into HindIII/SacI site
|
This study
|
|
pTRK1119
|
pTRK935 harboring a mutated copy of lba1426 gene cloned into HindIII/SacI site
|
This study
|
|
pTRK1126
|
pTRK935 harboring a mutated copy of lba0046 gene cloned into HindIII/SacI site
|
This study
|
|
pTRK1170
|
pTRK935 harboring a mutated copy of lba1426 gene cloned into BamHI/SacI site
|
This study
|
