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Table 1 Bacterial strains and plasmids used in this study

From: S-layer associated proteins contribute to the adhesive and immunomodulatory properties of Lactobacillus acidophilus NCFM

Strain or Plasmid Genotype or characteristics Reference
Strains
Lactobacillus acidophilus
  NCK56 (NCFM) Human intestinal isolate [28]
  NCK1909 (Δupp) NCFM with a 315 bp in-frame deletion within the upp gene (lba0770); background/parent strain for NCFM deletion mutants [27]
  NCK1910 NCK1909 harboring the repA helper plasmid pTRK669; host for pORI-based counterselective integration vector [27]
  NCK2439 (Δlba0864) NCK1909 with a 1425 bp in-frame deletion within the lba0864 gene This study
  NCK2441 (Δlba1426) NCK1909 with a 717 bp in-frame deletion within the lba1426 gene This study
  NCK2530 (Δlba0046) NCK1909 with a 342 bp in-frame deletion within the lba0046 gene This study
  NCK2608 (Δlba1539) NCK1909 with a 498 bp in-frame deletion within the lba1539gene This study
Escherichia coli
  EC101 RepA+ JM101; Kmr; repA from pWV01 integrated in chromosome; cloning host for pORI-based plasmids [29]
  NCK1391 E. coli DH10B harboring pTRK669; cloning host for pORI-based plasmids [27]
  NCK1911 EC101 host harboring pTRK935 integration vector [27]
  NCK2438 EC101 host harboring pTRK1118 recombinant plasmid This study
  NCK2440 EC101 host harboring pTRK1119 recombinant plasmid This study
  NCK2529 EC101 host harboring pTRK1126 recombinant plasmid This study
  NCK2607 DH10B host harboring pTRK1170 recombinant plasmid This study
Plasmids
 pTRK669 Ori (pWV01), Cmr, RepA+, thermosensitive [30]
 pTRK935 pORI upp-based counterselective integration vector, Emr [27]
 pTRK1118 pTRK935 harboring a mutated copy of lba0864 gene cloned into HindIII/SacI site This study
 pTRK1119 pTRK935 harboring a mutated copy of lba1426 gene cloned into HindIII/SacI site This study
 pTRK1126 pTRK935 harboring a mutated copy of lba0046 gene cloned into HindIII/SacI site This study
 pTRK1170 pTRK935 harboring a mutated copy of lba1426 gene cloned into BamHI/SacI site This study