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Table 1 Date of collection and location of positive flavivirus and phenuivirus-like sequence detection in mosquitoes from Mozambique, and their respective accession numbers

From: A survey of RNA viruses in mosquitoes from Mozambique reveals novel genetic lineages of flaviviruses and phenuiviruses, as well as frequent flavivirus-like viral DNA forms in Mansonia

Date of collection Locality/District Province Geo - references Pool lab code (size) Mosquito speciesa Flavivirus sequence Phenuivirus sequence COX1 mosquito sequence
03/12/14 Chinuara/ Mopeia Zambézia 17° 79′ 04. 748″ S; 035° 4′ 1442″ E Moz 3 (n = 50) Mansonia africana LC462008 LC517270
04/12/14 Chinuara/ Mopeia Zambézia 17° 79′ 04. 748″ S; 035° 4′ 1442″ E Moz 9 (n = 55) Mansonia africana LC462001 LC517271
27/04/15 Chinuara/ Mopeia Zambézia 17° 79′ 04. 748″ S; 035° 4′ 1442″ E Moz 19 (n = 50) Mansonia uniformis LC462246 n.a.
04/06/15 Limpopo park/ Massingir Gaza 23° 52′ 04.4399″ S; 032° 08′ 43.0852″ E Moz 38 (n = 20) Anopheles sp. LC462010 LC517272
26/11/14 Limpopo park/ Massingir Gaza 23° 52′ 04.4399″ S; 032° 08′ 43.0852″ E Moz 39 (n = 14) Anopheles sp. LC462009 LC517273
18/11/14 Goba/Namaacha Maputo 26° 3′ 59.73″ S; 032° 10′ 23.36″ E Moz 47 (n = 48) Anopheles coustani LC461999 LC517274
19/12/14 Goba/Namaacha Maputo 26° 3′ 59.73″ S; 032° 10′ 23.36″ E Moz 54 (n = 39) Culex tritaeniorhynchus LC461994 LC517275
LC461995
28/01/15 Goba/Namaacha Maputo 26° 3′ 59.73″ S; 032° 10′ 23.36″ E Moz 76 (n = 40) Mansonia uniformis LC462253 LC517276
28/01/15 Goba/Namaacha Maputo 26° 3′ 59.73″ S; 032° 10′ 23.36″ E Moz 77 (n = 64) Mansonia africana LC462249 LC517277
27/04/15 Chinuara/ Mopeia Zambézia 17° 79′ 04. 748″ S; 035° 4′ 1442″ E Moz 89 (n = 50) Mansonia africana LC462005 LC517278
27/04/15 Chinuara/ Mopeia Zambézia 17° 79′ 04. 748″ S; 035° 4′ 1442″ E Moz 90 (n = 50) Mansonia uniformis LC462251 LC517279
01/10/15 Goba/Namaacha Maputo 26° 3′ 59.73″ S; 032° 10′ 23.36″ E Moz 96 (n = 50) Mansonia africana LC462016 LC517280
21/07/15 Goba/Namaacha Maputo 26° 3′ 59.73″ S; 032° 10′ 23.36″ E Moz 97 (n = 12) Anopheles coustani LC462000 LC517281
21/07/15 Goba/Namaacha Maputo 26° 3′ 59.73″ S; 032° 10′ 23.36″ E Moz 98 (n = 18) Anopheles coustani LC462013 LC517282
28/01/15 Goba/Namaacha Maputo 26° 3′ 59.73″ S; 032° 10′ 23.36″ E Moz 104 (n = 53) Mansonia uniformis LC462007 LC517283
27/04/15 Chinuara/ Mopeia Zambézia 17° 79′ 04. 748″ S; 035° 4′ 1442″ E Moz 160 (n = 50) Anopheles pretoriensis LC462011 LC461996 LC517284
LC461997
27/04/15 Chinuara/ Mopeia Zambézia 17° 79′ 04. 748″ S; 035° 4′ 1442″ E Moz 161 (n = 49) Anopheles pretoriensis LC462012 LC517285
09/01/15 Chinuara/ Mopeia Zambézia 17° 79′ 04. 748″ S; 035° 4′ 1442″ E Moz 166 (n = 26) Anopheles pretoriensis LC462015 LC461998 LC517286
09/01/15 Chinuara/ Mopeia Zambézia 17° 79′ 04. 748″ S; 035° 4′ 1442″ E Moz 182 (n = 50) Culex antennatus LC462017 LC517287
09/01/15 Chinuara/ Mopeia Zambézia 17° 79′ 04. 748″ S; 035° 4′ 1442″ E Moz 212 (n = 50) Culex antennatus LC462254 LC517288
LC462255
03/12/14 Chinuara/ Mopeia Zambézia 17° 79′ 04. 748″ S; 035° 4′ 1442″ E Moz 258 (n = 50) Mansonia africana LC462003 LC517289
03/12/14 Chinuara/ Mopeia Zambézia 17° 79′ 04. 748″ S; 035° 4′ 1442″ E Moz 259 (n = 54) Mansonia africana LC462002 n.a.
03/12/14 Chinuara/ Mopeia Zambézia 17° 79′ 04. 748″ S; 035° 4′ 1442″ E Moz 263 (n = 75) Mansonia africana LC462004 n.a.
03/12/14 Chinuara/ Mopeia Zambézia 17° 79′ 04. 748″ S; 035° 4′ 1442″ E Moz 269 (n = 51) Mansonia africana LC462006 n.a.
09/01/15 Chinuara/ Mopeia Zambézia 17° 79′ 04. 748″ S; 035° 4′ 1442″ E Moz 299 (n = 45) Mansonia uniformis LC462250 LC517290
LC462256
20/12/14 Goba/Namaacha Maputo 26° 3′ 59.73″ S; 032° 10′ 23.36″ E Moz 309 (n = 8) Mansonia uniformis LC462252 n.a.
30/07/15 Chinuara/ Mopeia Zambézia 17° 79′ 04. 748″ S; 035° 4′ 1442″ E Moz 313 (n = 50) Mansonia africana LC462247 LC517291
LC462257
30/07/15 Chinuara/ Mopeia Zambézia 17° 79′ 04. 748″ S; 035° 4′ 1442″ E Moz 319 (n = 50) Mansonia africana LC462014 LC517292
26/06/15 Chinuara/ Mopeia Zambézia 17° 79′ 04. 748″ S; 035° 4′ 1442″ E Moz 324 (n = 1) Mansonia uniformis LC462019 LC517293
03/12/14 Chinuara/ Mopeia Zambézia 17° 79′ 04. 748″ S; 035° 4′ 1442″ E Moz 341 (n = 5) Coquillettidia metallica LC462018 LC536568
  1. aIdentification to the species level was confirmed based on COX1 barcoding using sequences generated by Sanger (population) sequencing of PCR products, except where marked with “n.a.” in COX1 mosquito sequence accession numbers column