A survey of RNA viruses in mosquitoes from Mozambique reveals novel genetic lineages of flaviviruses and phenuiviruses, as well as frequent flavivirus-like viral DNA forms in Mansonia

Abílio, Ana Paula; Silva, Manuel; Kampango, Ayubo; Narciso, Inácio; Gudo, Eduardo Samo; das Neves, Luís Carlos Bernardo; Sidat, Mohsin; Fafetine, José Manuel; de Almeida, António Paulo Gouveia; Parreira, Ricardo

doi:10.1186/s12866-020-01905-5

Table 1 Date of collection and location of positive flavivirus and phenuivirus-like sequence detection in mosquitoes from Mozambique, and their respective accession numbers

From: A survey of RNA viruses in mosquitoes from Mozambique reveals novel genetic lineages of flaviviruses and phenuiviruses, as well as frequent flavivirus-like viral DNA forms in Mansonia

Date of collection	Locality/District	Province	Geo - references	Pool lab code (size)	Mosquito species^a	Flavivirus sequence	Phenuivirus sequence	COX1 mosquito sequence
03/12/14	Chinuara/ Mopeia	Zambézia	17° 79′ 04. 748″ S; 035° 4′ 1442″ E	Moz 3 (n = 50)	Mansonia africana	LC462008	–	LC517270
04/12/14	Chinuara/ Mopeia	Zambézia	17° 79′ 04. 748″ S; 035° 4′ 1442″ E	Moz 9 (n = 55)	Mansonia africana	LC462001	–	LC517271
27/04/15	Chinuara/ Mopeia	Zambézia	17° 79′ 04. 748″ S; 035° 4′ 1442″ E	Moz 19 (n = 50)	Mansonia uniformis	LC462246	–	n.a.
04/06/15	Limpopo park/ Massingir	Gaza	23° 52′ 04.4399″ S; 032° 08′ 43.0852″ E	Moz 38 (n = 20)	Anopheles sp.	LC462010	–	LC517272
26/11/14	Limpopo park/ Massingir	Gaza	23° 52′ 04.4399″ S; 032° 08′ 43.0852″ E	Moz 39 (n = 14)	Anopheles sp.	LC462009	–	LC517273
18/11/14	Goba/Namaacha	Maputo	26° 3′ 59.73″ S; 032° 10′ 23.36″ E	Moz 47 (n = 48)	Anopheles coustani	–	LC461999	LC517274
19/12/14	Goba/Namaacha	Maputo	26° 3′ 59.73″ S; 032° 10′ 23.36″ E	Moz 54 (n = 39)	Culex tritaeniorhynchus	–	LC461994	LC517275
19/12/14	Goba/Namaacha	Maputo	26° 3′ 59.73″ S; 032° 10′ 23.36″ E	Moz 54 (n = 39)	Culex tritaeniorhynchus	–	LC461995	LC517275
28/01/15	Goba/Namaacha	Maputo	26° 3′ 59.73″ S; 032° 10′ 23.36″ E	Moz 76 (n = 40)	Mansonia uniformis	LC462253	–	LC517276
28/01/15	Goba/Namaacha	Maputo	26° 3′ 59.73″ S; 032° 10′ 23.36″ E	Moz 77 (n = 64)	Mansonia africana	LC462249	–	LC517277
27/04/15	Chinuara/ Mopeia	Zambézia	17° 79′ 04. 748″ S; 035° 4′ 1442″ E	Moz 89 (n = 50)	Mansonia africana	LC462005	–	LC517278
27/04/15	Chinuara/ Mopeia	Zambézia	17° 79′ 04. 748″ S; 035° 4′ 1442″ E	Moz 90 (n = 50)	Mansonia uniformis	LC462251	–	LC517279
01/10/15	Goba/Namaacha	Maputo	26° 3′ 59.73″ S; 032° 10′ 23.36″ E	Moz 96 (n = 50)	Mansonia africana	LC462016	–	LC517280
21/07/15	Goba/Namaacha	Maputo	26° 3′ 59.73″ S; 032° 10′ 23.36″ E	Moz 97 (n = 12)	Anopheles coustani	–	LC462000	LC517281
21/07/15	Goba/Namaacha	Maputo	26° 3′ 59.73″ S; 032° 10′ 23.36″ E	Moz 98 (n = 18)	Anopheles coustani	LC462013	–	LC517282
28/01/15	Goba/Namaacha	Maputo	26° 3′ 59.73″ S; 032° 10′ 23.36″ E	Moz 104 (n = 53)	Mansonia uniformis	LC462007	–	LC517283
27/04/15	Chinuara/ Mopeia	Zambézia	17° 79′ 04. 748″ S; 035° 4′ 1442″ E	Moz 160 (n = 50)	Anopheles pretoriensis	LC462011	LC461996	LC517284
27/04/15	Chinuara/ Mopeia	Zambézia	17° 79′ 04. 748″ S; 035° 4′ 1442″ E	Moz 160 (n = 50)	Anopheles pretoriensis	LC462011	LC461997	LC517284
27/04/15	Chinuara/ Mopeia	Zambézia	17° 79′ 04. 748″ S; 035° 4′ 1442″ E	Moz 161 (n = 49)	Anopheles pretoriensis	LC462012	–	LC517285
09/01/15	Chinuara/ Mopeia	Zambézia	17° 79′ 04. 748″ S; 035° 4′ 1442″ E	Moz 166 (n = 26)	Anopheles pretoriensis	LC462015	LC461998	LC517286
09/01/15	Chinuara/ Mopeia	Zambézia	17° 79′ 04. 748″ S; 035° 4′ 1442″ E	Moz 182 (n = 50)	Culex antennatus	LC462017	–	LC517287
09/01/15	Chinuara/ Mopeia	Zambézia	17° 79′ 04. 748″ S; 035° 4′ 1442″ E	Moz 212 (n = 50)	Culex antennatus	LC462254	–	LC517288
09/01/15	Chinuara/ Mopeia	Zambézia	17° 79′ 04. 748″ S; 035° 4′ 1442″ E	Moz 212 (n = 50)	Culex antennatus	LC462255	–	LC517288
03/12/14	Chinuara/ Mopeia	Zambézia	17° 79′ 04. 748″ S; 035° 4′ 1442″ E	Moz 258 (n = 50)	Mansonia africana	LC462003	–	LC517289
03/12/14	Chinuara/ Mopeia	Zambézia	17° 79′ 04. 748″ S; 035° 4′ 1442″ E	Moz 259 (n = 54)	Mansonia africana	LC462002	–	n.a.
03/12/14	Chinuara/ Mopeia	Zambézia	17° 79′ 04. 748″ S; 035° 4′ 1442″ E	Moz 263 (n = 75)	Mansonia africana	LC462004	–	n.a.
03/12/14	Chinuara/ Mopeia	Zambézia	17° 79′ 04. 748″ S; 035° 4′ 1442″ E	Moz 269 (n = 51)	Mansonia africana	LC462006	–	n.a.
09/01/15	Chinuara/ Mopeia	Zambézia	17° 79′ 04. 748″ S; 035° 4′ 1442″ E	Moz 299 (n = 45)	Mansonia uniformis	LC462250	–	LC517290
09/01/15	Chinuara/ Mopeia	Zambézia	17° 79′ 04. 748″ S; 035° 4′ 1442″ E	Moz 299 (n = 45)	Mansonia uniformis	LC462256	–	LC517290
20/12/14	Goba/Namaacha	Maputo	26° 3′ 59.73″ S; 032° 10′ 23.36″ E	Moz 309 (n = 8)	Mansonia uniformis	LC462252	–	n.a.
30/07/15	Chinuara/ Mopeia	Zambézia	17° 79′ 04. 748″ S; 035° 4′ 1442″ E	Moz 313 (n = 50)	Mansonia africana	LC462247	–	LC517291
30/07/15	Chinuara/ Mopeia	Zambézia	17° 79′ 04. 748″ S; 035° 4′ 1442″ E	Moz 313 (n = 50)	Mansonia africana	LC462257	–	LC517291
30/07/15	Chinuara/ Mopeia	Zambézia	17° 79′ 04. 748″ S; 035° 4′ 1442″ E	Moz 319 (n = 50)	Mansonia africana	LC462014	–	LC517292
26/06/15	Chinuara/ Mopeia	Zambézia	17° 79′ 04. 748″ S; 035° 4′ 1442″ E	Moz 324 (n = 1)	Mansonia uniformis	LC462019	–	LC517293
03/12/14	Chinuara/ Mopeia	Zambézia	17° 79′ 04. 748″ S; 035° 4′ 1442″ E	Moz 341 (n = 5)	Coquillettidia metallica	LC462018	–	LC536568

^aIdentification to the species level was confirmed based on COX1 barcoding using sequences generated by Sanger (population) sequencing of PCR products, except where marked with “n.a.” in COX1 mosquito sequence accession numbers column

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