Fig. 2

a Phylogenetic analysis of flavivirus NS5 nucleotide sequences (≈1 kbp per sequence). At specific branches, the number of “*” indicates the branch-support as revealed by the different phylogenetic reconstructions methods used, and assuming as relevant bootstrap values ≥75% (using 1000 resamplings of the sequence data in maximum likelihood analysis) and posterior probability values ≥0.80 (when Bayesian approaches were used). One, two or three “*” would indicate that a given branch had been supported by one, two, or all the phylogenetic reconstruction approaches used in the amalysis (ML and Bayesian analysis using two sets of demographic priors). At the top of the tree, the collapsed monophyletic group including reference sequences from mosquito-borne viruses (MBV), tick-borne viruses (TBV), no known vector viruses (NKV), and dual-host associated insect-specific viruses (dISF), while the branches shown comprise the so-called classical insect-specific flaviviruses (cISF), is expanded at the right (b). The sequences described in this work are indicated in bold-face. All the sequences used are designated by their respective accession numbers|virus name. The size bar indicates the number of nucleotide substitutions per site. §-Mosquito species could not be confirmed by COI sequence