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Fig. 3 | BMC Microbiology

Fig. 3

From: Antimicrobial and immunomodulatory responses of photodynamic therapy in Galleria mellonella model

Fig. 3

Hemocytes from ALA-PDT treated larvae do not contribute to the killing of S. aureus and C. albicans in vitro.a The disk diffusion assay against S. aureus and C. albicans. The larval hemolymph (containing hemocytes) was collected after ALA-mediated PDT treatment. PBS, negative control was the blank paper disk impregnated with PBS. GM or ITZ, positive control was the blank paper disk impregnated with gentamicin (GM, 1 mg/mL) or itraconazole (ITZ, 16 mg/L). b Kinetics of bacterial and fungal killing by insect hemocytes. Five larvae were treated with ALA-PDT. PBS was used as control. The hemocytes were collected and pooled. S. aureus or C. albicans cells were exposed to hemocytes at the indicated timepoints. The data are from a representative experiment performed in triplicate

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