Table 3 PCR primers used in this study
Gene | Primer | Primer sequence 5′➔3′ | Reference |
|---|---|---|---|
nuc | nuc-F | GCGATTGATGGTGATACGGTT | 44 |
nuc-R | AGCCAAGCCTTGACGAACTAAAGC | ||
mecA | mecA147-F | GTGAAGATATACCAAGTGATT | 45 |
mecA147-R | ATGCGCTATAGATTGAAAGGAT | ||
spa | 1095-F | AGACGATCCTTCGGTGAGC | 48 |
1517-R | GCTTTTGCAATGTCATTTACTG | ||
SCCmec | Type II-F | CGTTGAAGATGATGAAGCG | 46, 47 |
Type II-R | CGAAATCAATGGTTAATGGACC | ||
Type-IIb-F | TAGCTTATGGTGCTTATGCG | ||
Type-IIb-R | GTGCATGATTTCATTTGTGGC | ||
Type-IVa-F | GCCTTATTCGAAGAAACCG | ||
Type-IVa-R | CTACTCTTCTGAAAAGCGTCG | ||
Type IVE-F | CAGATTCATCATTTCAAAGGC | ||
Type IVE-R | AACAACTATTAGATAATTTCCG | ||
Type IVc-F | CCTGAATCTAAAGAGATACACCG | ||
Type IVc-R | GGTTATTTTCATAGTGAATCGC | ||
arcC | arcC-F | TTG ATT CAC CAG CGC GTA TTG TC | 50 |
arcC-R | AGG TAT CTG CTT CAA TCA GCG | ||
aro | aro-F | ATC GGA AAT CCT ATT TCA CAT TC | |
aro-R | GGT GTT GTA TTA ATA ACG ATA TC | ||
glp | glp-F | CTA GGA ACT GCA ATC TTA ATC C | |
glp-R | TGG TAA AAT CGC ATG TCC AAT TC | ||
gmk | gmk-F | ATC GTT TTA TCG GGA CCA TC | |
gmk-R | TCATTAACTACAACGTAATCGTA | ||
pta | pta-F | GTTAAAATCGTATTACCTGAAGG | |
pta-R | GACCCTTTTGTTGAAAAGCTTAA | ||
tpi | tpi-F | TCGTTCATTCTGAACGTCGTGAA | |
tpi-R | TTTGCACCTTCTAACAATTGTAC | ||
yqi | yqi-F | CAGCATACAGGACACCTATTGGC | |
yqi-R | CGTTGAGGAATCGATACTGGAAC |