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Table 5 Proportion (% of total intermediates and 18:2n-6) of 18:2n-6 and its biohydrogenation intermediates after 24 h of incubation under different growth conditionsa with different biomass ratios of Butyrivibrio fibrisolvens D1 to Propionibacterium acnes DSM 1897 in the inoculum (Exp. 4)

From: Identifying and exploring biohydrogenating rumen bacteria with emphasis on pathways including trans-10 intermediates

 

B. fibrisolvens (%)/C. acnes (%)

SEM

P-value

100/0

50/50

10/90

2/98

0.4/99.6

0/100

b

Linearc

Quadraticd

18:2n-6

Control

1.81

2.18

4.76

25.95

16.44

46.67

13.050

R***

<  0.001

0.004

Low pH

53.91*

60.34*

53.52*

48.95*

51.49*

55.24

M***

0.579

0.320

DHA

13.28

20.29

38.47*

57.28*

64.67*

75.28*

R × M***

<  0.001

0.014

c9, t11 CLAe

Control

0.20

18.28

14.75

7.78

5.03

<  0.01

7.534

R***

<  0.001

0.004

Low pH

45.51*

17.59

4.03

0.95

<  0.01

<  0.01

M***

<  0.001

0.563

DHA

82.52*

70.06*

38.85*

18.20

9.79

<  0.01

R × M***

0.828

0.043

trans-11 18:1

Control

97.93

72.57

57.69

31.88

44.80

0.46

9.460

R**

0.772

0.977

Low pH

0.57*

0.38*

0.37*

0.44*

0.42*

0.39

M***

0.988

0.990

DHA

4.11*

2.15*

2.28*

0.74*

2.36*

0.36

R × M***

<  0.001

0.086

t10, c12 CLAf

Control

<  0.01

2.63

8.72

18.98

13.61

53.37

6.606

R***

<  0.001

0.629

Low pH

<  0.01

21.70*

42.04*

49.66*

48.01*

44.37

M***

<  0.001

0.767

DHA

<  0.01

8.15

19.97

23.57

21.69

24.29*

R × M**

<  0.001

0.019

trans-10 18:1

Control

0.06

4.34

14.08

15.40

20.11

<  0.01

3.927

R*

0.905

0.943

Low pH

<  0.01

<  0.01

0.04*

<  0.01*

0.08*

<  0.01

M***

0.992

0.998

DHA

0.08

<  0.01

0.42*

0.21*

0.90*

0.06

R × M*

<  0.001

0.911

trans-10/trans-11g

Control

–

0.08

0.34

2.46

2.44

–

9.296

R***

0.017

0.082

Low pH

–

1.22

12.67

65.74*

90.01*

–

M***

<  0.001

<  0.001

DHA

–

0.10

0.95

2.86

9.74

–

R × M**

<  0.001

0.003

  1. a Low pH, control medium with pH adjusted to 5.5; DHA (docosahexaenoic acid), control medium containing 40 μg/mL of 22:6n-3; all growth media contained 40 μg/mL of 18:2n-6
  2. b R, effect of ratio of B. fibrisolvens to C. acnes in the inoculum; M, effect of growth medium; * 0.01 ≤ P < 0.05; ** 0.001 ≤ P < 0.01; *** P < 0.001
  3. c Linear effect of R within each growth medium
  4. d Quadratic effect of R within each growth medium
  5. ec9, t11 CLA, cis-9, trans-11 conjugated linoleic acid
  6. ft10, c12 CLA, trans-10, cis-12 conjugated linoleic acid
  7. g Ratio of trans-10 intermediates (trans-10, cis-12 CLA + trans-10 18:1) to trans-11 intermediates (cis-9, trans-11 CLA + trans-11 18:1)
  8. * Means differ (P < 0.05) from the control growth medium within the same ratio
  9. -: The ratio is not relevant for mono-cultures as trans-11 and trans-10 are produced exclusively with mono-cultures of B. fibrisolvens and C. acnes, respectively
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BMC Microbiology

ISSN: 1471-2180

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