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Table 4 Average proportionsa (% of total intermediates and 18:2n-6) of 18:2n-6b and its biohydrogenation intermediates over a 24 h incubation period with different ratios of Butyrivibrio fibrisolvens D1 to Propionibacterium acnes DSM 1897 in the inoculum under control growth conditions (Exp. 4)

From: Identifying and exploring biohydrogenating rumen bacteria with emphasis on pathways including trans-10 intermediates

 

B. fibrisolvens (%)/C. acnes (%)

SEM

P-value

100/0

50/50

10/90

2/98

0.4/99.6

0/100

Linear

Quadratic

18:2n-6

12.35

19.59

34.90

49.57

53.30

66.65

12.928

<  0.001

<  0.001

cis-9, trans-11 CLAc

19.22

22.44

10.57

8.89

4.15

0.02

7.797

0.013

0.090

trans-11 18:1

67.98

51.72

34.59

14.22

15.62

0.41

14.947

<  0.001

0.035

trans-10, cis-12 CLAc

<  0.01

3.03

11.55

20.08

19.13

32.92

3.123

<  0.001

0.007

trans-10 18:1

0.45

3.22

8.39

7.24

7.80

<  0.01

4.090

0.014

0.471

trans-10/trans-11d

–

0.08

0.48

3.00

4.04

–

4.436

<  0.001

<  0.001

  1. a The average proportions of FA over the 24 h incubation period were computed as the area under the curve divided by the total duration of incubation (24 h), using the individual measured proportions for each FA at the different sampling times
  2. b The initial amount of 18:2n-6 was 40 μg/mL
  3. c CLA, conjugated linoleic acid
  4. d Ratio of trans-10 intermediates (trans-10, cis-12 CLA + trans-10 18:1) to trans-11 intermediates (cis-9, trans-11 CLA + trans-11 18:1)
  5. -: The ratio is not relevant for mono-cultures as trans-11 and trans-10 are produced exclusively with mono-cultures of B. fibrisolvens and C. acnes, respectively
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BMC Microbiology

ISSN: 1471-2180

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