Table 1 Blocking and excluding primers used for metabarcoding analyses
Marker region | Target | Forward (5′- > 3′) | Reverse (5′- > 3′) | Blocking primer (5′- > 3′) |
|---|---|---|---|---|
18SV4 | Eukaryota | CCAGCASCYGCGGTAATTCC | ACTTTCGTTCTTGATYRA | TCTTGACTAATGAAAACATGCTTGG |
18SV1V2 | Non-metazoa | ACCTGGTTGATCCTGCCA | GTARKCCWMTAYMYTACC | No blocking primer |