Fig. 5
From: The effects of clioquinol in morphogenesis, cell membrane and ion homeostasis in Candida albicans
The influence of clioquinol on ion homeostasis. The optical density (OD) at 600 nm of fungal cells in RMPI 1640 medium without addition of clioquinol, exogenous metal ions, exogenous metal chelators was used as standard value in (a to e). The OD values of experiment groups were compared to standard value to obtain the relative value. a The antifungal activity of clioquinol and metal chelators; b The influence of exogenous metal ions in antifungal activity of 1μM clioquinol; c The influence of exogenous metal ions in antifungal activity of 5 μM clioquinol; d The influence of exogenous metal ions in MIC value of clioquinol; e The influence of exogenous metal chelators in MIC value of clioquinol; f Cellular labile ferrous iron level in clioquinol-treated fungal cells. Fluorescence intensity of fungal cells in YPD medium without addition of clioquinol, exogenous metal ions, exogenous metal chelators was used as standard value and the fluorescence intensities of experiment groups was compared to standard value to obtain the relative value. MIC, the minimum inhibitory concentration; EDTA, ethylenediamine tetraacetic acid; TPEN, N,N,N′,N′-Tetrakis-(2-pyridylmethyl) ethylenediamine; BPS, basophenanthrolinedisulfonate disodium salt; YPD: Yeast peptone dextrose; ***, p < 0.0001