Fig. 4

The effect of 17ß-estradiol on the yeast-sensitivity of Culex quinquefasciatus mosquitoes. a Multiple amino acid sequence alignment between D. melanogaster Estrogen Related Receptor (dmERR) and C. quinquefasciatus ERR (cqERR). The sequences of dmERR (Accession NP_648183) and cqERR (Accession EDS37237) were aligned using MultAlin software as in Fig. 1b. The DNA binding domain and ligand binding domain are highlighted in blue and green boxes, respectively. Identical amino acids are shown in red. The extent (%) of the identity/similarity between Drosophila and Culex sequences is shown above the alignment. b Female C. quinquefasciatus mosquitoes were orally challenged with 3.3 × 10⁷S. cerevisiae cells/ml with and without 75 mM of 17ß-estradiol (17ß-E). P-value indicates statistical significance compared to the yeast-only condition (asterisks) on the basis of the Log-rank (Mantel-Cox) test