Fig. 3

Steroid molecules increase the sensitivity of female wildtype flies to Saccharomyces cerevisiae. a Structures of steroid skeleton and cortisol analogs tested in this study: cortisone acetate, 17ß-estradiol, and ergosterol. (b-c) Female wild type (WT) flies were orally challenged as in Fig. 1 with 1.67 × 10⁷S. cerevisiae cells/ml with and without various concentrations of cortisone acetate (CA) (b) and 17ß-estradiol (17ß-E) (c). Uninfected flies exposed to 100 mM cortisone acetate or 75 mM 17ß-estradiol were included to test the toxicity of these compounds. P-values in b-c indicate statistical significance compared to the yeast-only condition (asterisks) on the basis of the Log-rank (Mantel-Cox) test