Skip to main content

Table 1 Primers and conditions used in qPCR quantification of microbial cultures

From: Biofilm growth and IL-8 & TNF-α-inducing properties of Candida albicans in the presence of oral gram-positive and gram-negative bacteria

Species

Primer sequence 5′-3′

Annealing temperatures

(°C)

References

A. actinomycetemcomitans

F: TAGCCCTGGTGCCCGAAGC

R: CATCGCTGGTTGGTTACCCTCTG

68

[61]

S. mutans

F: TCGCGAAAAAGATAAACAAACA

R: GCCCCTTCACAGTTGGTTAG

56

[62]

S. gordonii

F: GGTGTTGTTTGACCCGTTCAG

R: AGTCCATCCCACGAGCACAG

53

[63]

C. albicans

F: TCA ACT TGT CAC ACC AGA TTA TT

R: TCC TCC GCT TAT TGA TAT GC

52

[64]