Fig. 7

Targeted mutagenesis against the crotonyl-CoA carboxylase/reductase gene (ccr). The growth of the Rs. rubrum S1H ∆ccr::Km^R (green; n = 5) and wild-type (gray; n = 5) strains was monitored by measuring the OD_680nm under light anaerobic conditions with succinate (opened markers) or butyrate (filled markers) as the carbon source and supplemented with 50 mM bicarbonate. Red arrows represent bicarbonate supplementation (NaHCO_3, 180 μmoles)