Table 1 Detection of tet(X3) and tet(X4) genes in isolates
Isolate | Origin | Species | Gene | Gene Location | Real-time PCR for tet(X) | ||
|---|---|---|---|---|---|---|---|
tet(X3) | tet(X4) | Ct ± SD | |||||
ATCC25922 | – | E. coli | – | – | – | – | Undetermined |
DH5α-tet(X) | – | E. coli | tet(X) | Plasmid | – | – | Undetermined |
DH5α-tet(X1) | – | E. coli | tet(X1) | Plasmid | – | – | Undetermined |
DH5α-tet(X2) | – | E. coli | tet(X2) | Plasmid | – | – | Undetermined |
DH5α-tet(X3) | – | E. coli | tet(X3) | Plasmid | + | – | 9.66 ± 0.00 |
DH5α-tet(X4) | – | E. coli | tet(X4) | Plasmid | – | + | 9.27 ± 0.00 |
CB13 | Chicken | E. coli | tet(X3) | Plasmid | + | – | 19.05 ± 0.05 |
CB14 | Chicken | E. coli | tet(X3) | Plasmid | + | – | 21.06 ± 0.08 |
CB15 | Chicken | E. coli | tet(X3) | Plasmid | + | – | 19.24 ± 0.10 |
CB42 | Chicken | E. coli | tet(X3) | Plasmid | + | – | 19.24 ± 0.16 |
DZ47 | Chicken | E. coli | tet(X4) | Plasmid | – | + | 21.90 ± 0.12 |
AZ28 | Chicken | E. coli | tet(X4) | Plasmid | – | + | 14.79 ± 0.01 |
DZ4R | Chicken | A. baumannii | tet(X4) | Plasmid | – | + | 13.42 ± 0.07 |
NM4 | Chicken | A. baumannii | tet(X4) | Plasmid | – | + | 14.23 ± 0.13 |
DZ27 | Chicken | E. coli | tet(X4) | Plasmid | – | + | 17.76 ± 0.13 |
DZ24 | Chicken | E. coli | tet(X4) | Plasmid | – | + | 14.35 ± 0.10 |
DZ65 | Chicken | A. baumannii | tet(X4) | Plasmid | – | + | 21.05 ± 0.05 |
DZ24 | Chicken | E. coli | tet(X4) | Plasmid | – | + | 18.33 ± 0.09 |