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Table 1 Detection of tet(X3) and tet(X4) genes in isolates

From: A TaqMan-based multiplex real-time PCR assay for the rapid detection of tigecycline resistance genes from bacteria, faeces and environmental samples

IsolateOriginSpeciesGeneGene LocationReal-time PCR for tet(X)
tet(X3)tet(X4)Ct ± SD
ATCC25922E. coliUndetermined
DH5α-tet(X)E. colitet(X)PlasmidUndetermined
DH5α-tet(X1)E. colitet(X1)PlasmidUndetermined
DH5α-tet(X2)E. colitet(X2)PlasmidUndetermined
DH5α-tet(X3)E. colitet(X3)Plasmid+9.66 ± 0.00
DH5α-tet(X4)E. colitet(X4)Plasmid+9.27 ± 0.00
CB13ChickenE. colitet(X3)Plasmid+19.05 ± 0.05
CB14ChickenE. colitet(X3)Plasmid+21.06 ± 0.08
CB15ChickenE. colitet(X3)Plasmid+19.24 ± 0.10
CB42ChickenE. colitet(X3)Plasmid+19.24 ± 0.16
DZ47ChickenE. colitet(X4)Plasmid+21.90 ± 0.12
AZ28ChickenE. colitet(X4)Plasmid+14.79 ± 0.01
DZ4RChickenA. baumanniitet(X4)Plasmid+13.42 ± 0.07
NM4ChickenA. baumanniitet(X4)Plasmid+14.23 ± 0.13
DZ27ChickenE. colitet(X4)Plasmid+17.76 ± 0.13
DZ24ChickenE. colitet(X4)Plasmid+14.35 ± 0.10
DZ65ChickenA. baumanniitet(X4)Plasmid+21.05 ± 0.05
DZ24ChickenE. colitet(X4)Plasmid+18.33 ± 0.09