Fig. 2
From: A novel gene expression system for Ralstonia eutropha based on the T7 promoter
Minimization of plasmid vector pBBR1-PT7-rfp(mini). a Different plasmids were constructed by deleting various sizes of DNA cassettes from plasmid pj5_00018. PCR were performed using pBBR1_2303F and pBBR1_2303 as primers, which indicated that different sequences were deleted successfully. b Profile of the functional minimized plasmid pBBR1-PT7-rfp(mini), also named pj5_00030. F: the primer pBBR1_2303F, R: the primer pBBR1_2303R