Large plasmids encoding antibiotic resistance and localized-like adherence in atypical enteropathogenic Escherichia coli strains

Table 1 Results of conjugation between LAL⁺ aEPEC strains and E. coli K-12 C600

aEPEC strain	Source	Serotype	Resistance profile^a	Size (MDa) of plasmid(s)^b	Presence (+) or absence (−) of traA	Resistance profile of transconjugant(s)	Size (MDa) of transconjugant plasmid(s)	LAL phenotype
A1	Diarrhea	O26:NM	–	~ 60, 4.6	–	–	–	–
A24	Diarrhea	O26:NM	Ap^r	98	+	Ap^r Nal^r	98	–
A14	Diarrhea	O26:HND	Km^r Nal^r	98, ~ 60	–	–	–	–
A72	Diarrhea	O26:HND	Km^r Nal^r	~ 60	–	–	–	–
A153	Diarrhea	O26:HND	–	98, < 4.6	+	–	–	–
A46	Diarrhea	O55:HND	Ap^r Km^r Tc^r	~ 60, 98	+	Ap^r Tc^r Nal^r	98	+
A76	Diarrhea	O85:H40	Ap^r Cm^r	~ 60	+	Ap^r Cm^r Nal^r	98	–
A17	Control	O105:H7	–	~ 60	+	–	–	–
A12	Control	O111:NM	Km^r Nal^r	75, 4.6	–	–	–	–
A60	Diarrhea	O119:H2	–	98	+	–	–	–
A66	Diarrhea	O119:HND	Ap^r Tc^r	98	+	Ap^r Tc^r Nal^r	98	+
A13	Diarrhea	O126:NM	–	65, < 4.6	–	–	–	–
A11	Diarrhea	O142:NM	–	65, 4.6	+	–	–	–
A104	Diarrhea	ONT:H18	Ap^r Tc^r	> 98	+	Ap^r Tc^r Nal^r	> 98	–
A106	Diarrhea	ONT:H18	Ap^r Cm^r Tc^r	> 98	+	Ap^r Tc^r Nal^r	> 98	–
A10	Diarrhea	ONT:HND	–	> 98	+	–	–	–
A102	Diarrhea	ONT:HND	Ap^r Km^r Tc^r	98	+	Ap^r Tc^r Nal^r	98	+
A128	Diarrhea	ONT:HND	Ap^r Tc^r	98	+	Ap^r Tc^r Nal^r	98	–

^aAp^r, ampicillin resistant; Km^r, kanamycin resistant; Cm^r, chloramphenicol resistant; Tc^r, tetracycline resistant; Nal^r, nalidicix acid resistant
^bThe size of plasmids was calculated based on relative migration of plasmids with known sizes contained in strain 39-R861 [24]

ISSN: 1471-2180