Fig. 6

GvpN is an active ATPase. a SDS-PAGE of the purified GvpN protein. b The enzymatic profiles of GvpN. The final amounts of recombinant GvpN in the 200-μL system are 0, 25, 50, 100 and 200 μg, respectively. The decrease in absorbance at 340 nm was monitored using a DU800 spectrophotometer. c The Hanes-Woolf plot of GvpN. d Multiple-sequence alignment of GvpN from different species of cyanobacteria. The alignment was performed with the program Multalin. All sequences were downloaded from the NCBI database with the following accession numbers: Anabaena sp. PCC 7120, WP_010996409; Calothrix sp. PCC 7103, WP_019492266; Nodularia spumigena CCY9414, AHJ27875; Microcystis aeruginosa PCC 7806, WP_002747926; Cylindrospermopsis raciborskii, WP_061547066; Raphidiopsis brookii D9, EFA73420; Oscillatoria sp. PCC 10802, WP_017715028; Arthrospira platensis, WP_006616595; Pseudanabaena sp. SR411, WP_094529416; Synechococcus sp. PCC 7502, WP_015167038