Fig. 2
From: LipF increases rifampicin and streptomycin sensitivity in a Mycobacterium tuberculosis surrogate
Ectopic expression of LipF in M. smegmatis. a Total RNA was extracted from pMV261-lipF transformants and RT-PCR assays were performed to confirm lipF expression in M. smegmatis. Lane 1 MW: 1 Kb plus DNA ladder. Lane 2–5: treatment with or without DNase and RT. Lane 6: negative control nuclease free water. Lane 7: positive control (pMV261-lipF DNA). b LipF protein (arrow) in M. smegmatis transformed with pMV261-lipF