Table 6 Comparison of bacterial identification methods showing percentage identity for complexity-reduced genotyping based on BLASTn alignment; whole-genome sequencing best matches using progressiveMauve [35, 36] alignment tool; and the best matches obtained with 16S rRNA gene alignment, including multiple results per sample with equal highest bitscore and percentage identity
From: Discovery of thermophilic Bacillales using reduced-representation genotyping for identification
Group | Sample name | Complexity-reduced genotyping best match | % ID | Whole genome sequencing best match result | Mauve mean similarity profile value | 16 s rRNA in silico best match result | % ID |
|---|---|---|---|---|---|---|---|
A | MR_LB_pH8 | Geobacillus thermodenitrificans strain OS27 | 99.64 | Geobacillus thermodenitrificans strain KCTC3902 | 13,618.31 | Geobacillus thermodenitrificans strain KCTC3902 | 100.00 |
Geobacillus sp. PA-3 GEPA3 | 100.00 | ||||||
Geobacillus thermodenitrificans NG80–2 | 100.00 | ||||||
B_mud_LB_pH8 | Geobacillus vulcani PSS1 | 99.40 | Geobacillus vulcani PSS1 | 12,550.22 | Geobacillus vulcani PSS1 | 100.00 | |
Geobacillus sp. FW23 | 100.00 | ||||||
B | NFOSA3 | Geobacillus galactosidasius strain DSM 18751 | 98.85 | Geobacillus galactosidasius strain DSM 18751 | 8003.04 | Geobacillus galactosidasius strain DSM 18751 | 100.00 |
DSP4a | Geobacillus lituanicus strain N-3 | 98.55 | Geobacillus lituanicus strain N-3 | 4299.05 | Geobacillus stearothermophilus strain FHS-PHGT51 | 100.00 | |
Geobacillus stearothermophilus strain DSM 458 | 100.00 | ||||||
Geobacillus stearothermophilus strain GS27 | 100.00 | ||||||
Geobacillus sp. Sah69 | 100.00 | ||||||
Geobacillus stearothermophilus ATCC 12980 | 100.00 | ||||||
Geobacillus stearothermophilus ATCC 7953 | 100.00 | ||||||
CHMud_LB_pH8 | Anoxybacillus sp. 103 | 98.43 | Anoxybacillus sp. 103 | 7800.76 | Anoxybacillus kamchatkensis strain G10 | 100.00 | |
Efil_LB_pH6.8_a | Anoxybacillus ayderensis strain AB04 | 98.40 | Anoxybacillus ayderensis strain AB04 | 10,221.31 | Anoxybacillus kamchatkensis strain G10 | 99.66 | |
MMMud_3_LB_pH8 | Geobacillus subterraneus PSS2 | 98.14 | Geobacillus subterraneus PSS2 | 3684.00 | Geobacillus icigianus strain G1w1 | 99.76 | |
Geobacillus subterraneus PSS2 N671 | 99.76 | ||||||
C | MCWH_LB_pH8 | Brevibacillus thermoruber PM1 N690 | 94.13 | Brevibacillus thermoruber 423 | 3758.04 | Brevibacillus thermoruber PM1 N690 | 99.35 |