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Table 6 Comparison of bacterial identification methods showing percentage identity for complexity-reduced genotyping based on BLASTn alignment; whole-genome sequencing best matches using progressiveMauve [35, 36] alignment tool; and the best matches obtained with 16S rRNA gene alignment, including multiple results per sample with equal highest bitscore and percentage identity

From: Discovery of thermophilic Bacillales using reduced-representation genotyping for identification

GroupSample nameComplexity-reduced genotyping best match% IDWhole genome sequencing best match resultMauve mean similarity profile value16 s rRNA in silico best match result% ID
AMR_LB_pH8Geobacillus thermodenitrificans strain OS2799.64Geobacillus thermodenitrificans
strain KCTC3902
13,618.31Geobacillus thermodenitrificans strain KCTC3902100.00
      Geobacillus sp.
PA-3 GEPA3
100.00
      Geobacillus thermodenitrificans NG80–2100.00
 B_mud_LB_pH8Geobacillus vulcani
PSS1
99.40Geobacillus vulcani
PSS1
12,550.22Geobacillus vulcani
PSS1
100.00
      Geobacillus sp.
FW23
100.00
BNFOSA3Geobacillus galactosidasius strain DSM 1875198.85Geobacillus galactosidasius strain DSM 187518003.04Geobacillus galactosidasius strain DSM 18751100.00
 DSP4aGeobacillus lituanicus
strain N-3
98.55Geobacillus lituanicus
strain N-3
4299.05Geobacillus stearothermophilus strain FHS-PHGT51100.00
      Geobacillus stearothermophilus strain DSM 458100.00
      Geobacillus stearothermophilus strain GS27100.00
      Geobacillus sp.
Sah69
100.00
      Geobacillus stearothermophilus ATCC 12980100.00
      Geobacillus stearothermophilus ATCC 7953100.00
 CHMud_LB_pH8Anoxybacillus sp. 10398.43Anoxybacillus sp. 1037800.76Anoxybacillus kamchatkensis strain G10100.00
 Efil_LB_pH6.8_aAnoxybacillus ayderensis
strain AB04
98.40Anoxybacillus ayderensis
strain AB04
10,221.31Anoxybacillus kamchatkensis strain G1099.66
 MMMud_3_LB_pH8Geobacillus subterraneus PSS298.14Geobacillus subterraneus PSS23684.00Geobacillus icigianus
strain G1w1
99.76
      Geobacillus subterraneus PSS2 N67199.76
CMCWH_LB_pH8Brevibacillus thermoruber
PM1 N690
94.13Brevibacillus thermoruber
423
3758.04Brevibacillus thermoruber
PM1 N690
99.35
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