Fig. 3
Growth curves of REL606 expressing hpaB variants in defined medium with 4HPA (a) or 3HPA (b) as sole carbon source. E. coli REL606 cells harboring expression constructs BL21(DE3)-derived hpaB [pHCE-IIB-HpaB(R379), 
]; REL606-derived hpaB [pHCE-IIB-HpaB(C379), 
]; mutant hpaBs [(pHCE-IIB-HpaB(G379), 
]; and pHCE-IIB-HpaB(S379), 
]. REL606 harboring the control plasmid (pHCE-IIB, 
) was used as control. The culture medium comprised defined medium supplemented with 0.76 g/L (5 mM) of 3HPA or 4HPA. Error bars denote standard deviation of the mean from three independent cultures