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Fig. 3 | BMC Microbiology

Fig. 3

From: A single amino acid substitution in aromatic hydroxylase (HpaB) of Escherichia coli alters substrate specificity of the structural isomers of hydroxyphenylacetate

Fig. 3

Growth curves of REL606 expressing hpaB variants in defined medium with 4HPA (a) or 3HPA (b) as sole carbon source. E. coli REL606 cells harboring expression constructs BL21(DE3)-derived hpaB [pHCE-IIB-HpaB(R379), ]; REL606-derived hpaB [pHCE-IIB-HpaB(C379), ]; mutant hpaBs [(pHCE-IIB-HpaB(G379), ]; and pHCE-IIB-HpaB(S379), ]. REL606 harboring the control plasmid (pHCE-IIB, ) was used as control. The culture medium comprised defined medium supplemented with 0.76 g/L (5 mM) of 3HPA or 4HPA. Error bars denote standard deviation of the mean from three independent cultures

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