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Table 3 Quantity and quality of DNA extracted using two DNA extraction methods

From: Optimizing 16S rRNA gene profile analysis from low biomass nasopharyngeal and induced sputum specimens

ControlDNA extraction kitBufferReplicate16S rRNA gene copy numbers (copies/ml of specimen input volume)260/280 NanoDrop® ND-1000 ratio
Zymobiomics-Primestore-highKit-QSPrimestore12.47E91.68
22.06E91.75
31.92E91.90
Kit-ZBPrimestore12.14E92.09
21.99E91.34
31.61E91.19
Zymobiomics-Primestore-lowKit-QSPrimestore13.77E3
25.82E3
35.37E3
Kit-ZBPrimestore12.08E5
22.72E5
33.43E5
Zymobiomics-STGG-highKit-QSSTGG17.52E81.96
27.32E81.89
35.58E81.93
Kit-ZBSTGG13.09E92.53
21.89E91.34
31.73E91.23
Zymobiomics-STGG-lowKit-QSSTGG12.79E5
23.58E5
35.10E5
Kit-ZBSTGG11.89E5
22.85E5
32.76E5
  1. Zymobiomics-Primestore-high 900 μl of Zymobiomics-Cells suspended in 3600 μl Primestore, Zymobiomics-Primestore-low 1-in-104 fold dilution of Zymobiomics-Primestore-high, Zymobiomics-STGG-high, 900 μl of Zymobiomics-Cells suspended in 3600 μl STGG, Zymobiomics-STGG-low 1-in-104 fold dilution of Zymobiomics-STGG-high, Kit-QS DSP Virus/Pathogen Mini Kit® using QIAsymphony® SP instrument (catalogue no. 937036, Qiagen GmbH, Hilden, Germany), Kit; ZB ZymoBIOMICS DNA Miniprep Kit (catalogue no. ZR D4300, Zymo Research Corp., Irvine, CA, United States), Primestore PrimeStore® Molecular Transport medium (Longhorn Vaccines & Diagnostics Bethesda, MD, USA), STGG Storage medium containing skim milk, tryptone, glucose, and glycerine