Fig. 3
From: The exopolysaccharide–eDNA interaction modulates 3D architecture of Bacillus subtilis biofilm
Dynamics of morphogenesis, three- dimensional (3D) architecture development and microbial population shifts of B. subtilis SBE1 biofilms. The biofilms stained with TOTO-1 for eDNA (green) and SYTO 60 for bacteria (red). Representative 3D rendering images of B. subtilis SBE1 biofilms at 12 h (a), 24 h (b) and 48 h (c). At the upper left of each panel, the two channels are displayed separately, while the merged image is displayed at the bottom right. A magnified (close-up) view of each small box depicted in the merged image is positioned in the upper right corner of each panel