Table 1 List of strains and plasmids used in this study
Strain or plasmid | Relevant characteristics | Source |
|---|---|---|
Escherichia coli | ||
DH5α | F−φ80 lacZΔM15Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rk-, mk+) phoA supE44 λ- thi-1 gyrA96 relA1 | Clontech |
X. citri pv. malvacearum | ||
MSCT1 | Wild-type, causes bacterial blight of cotton | [59] |
XcmH1005 | Spontaneous Rifr derivative of XcmN | [27] |
XcmH1003 | Spr, Rifr derivative of XcmN | [27] |
Xcc049 | Wild-type Xcc strain, used for construction of pKMSA1/A2 | This lab |
Xss-V2–18 | Wild-type, causes bacterial blight of cotton | Hainan University, China |
M1 | tal3 deletion mutant of Xss-V2–18 | This study |
M2 | tal2 deletion mutant of Xss-V2–18 | This study |
M3 | tal2 tal4 deletion mutant of Xss-V2–18 | This study |
M4 | tal2 tal4 tal5 tal6 deletion mutant of Xss-V2–18 | This study |
M5 | tal2 tal3 tal4 tal5 tal6 deletion mutant of Xss-V2–18 | This study |
M6 | tal-free mutant of Xss-V2–18 | This study |
Plasmids | ||
pBluescript II SK(−) | Apr, phagemid, pUC derivative | Lab collection |
pMD18-T | Apr, pUC18 derivative, TA cloning vector, 2692 bp | TaKaRa |
pKMS1 | Kmr, sacB mob lacZ oriV, 6475 bp | [60] |
pHM1 | Broad-spectrum cosmid vector, cos, parA, IncW, Spr | [61] |
pKMSA1 | pKMS1 containing a 930-bp XbaI/ SmaI fragment; insert contains a fusion of a and b fragments that encode the N- and C-terminal sides of the tal CRR; Kmr | This study |
pKMSA2 | pKMS1 containing a 450-bp XbaI/ SmaI fragment; insert contains a fusion of the c and d fragments that encode the N- and C-terminal sides of the tal CRR; Kmr | This study |
pB-tal1 | pBluescriptIISK(−) containing tal1 of Xss-V2–18 | This study |
pB-tal2 | pBluescript II SK(−) containing tal2 of Xss-V2–18 | This study |
pB-tal3 | pBluescript II SK(−) containing tal3 of Xss-V2–18 | This study |
pB-tal4 | pBluescript II SK(−) containing tal4 of Xss-V2–18 | This study |
pB-tal5 | pBluescript II SK(−) containing tal5 of Xss-V2–18 | This study |
pB-tal6 | pBluescript II SK(−) containing tal6 of Xss-V2–18 | This study |
pZWavrXa7 | avrXa7 in pBluescript II KS+, contains FLAG epitope immediately downstream of the second SphI site in the C-terminus of AvrXa7, Apr | [62] |
pZW-tal2 | SphI fragment of tal2 in pZWavrXa7, Apr | This study |
pHZW-tal2 | pHM1 fused with pZW-tal2 at HindIII, lacZ promoter upstream of tal2, Apr, Spr | This study |