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Table 1 List of strains and plasmids used in this study

From: Identification of a virulence tal gene in the cotton pathogen, Xanthomonas citri pv. malvacearum strain Xss-V2–18

Strain or plasmidRelevant characteristicsSource
Escherichia coli 
DH5αFφ80 lacZΔM15Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rk-, mk+) phoA supE44 λ- thi-1 gyrA96 relA1Clontech
X. citri pv. malvacearum
MSCT1Wild-type, causes bacterial blight of cotton[59]
XcmH1005Spontaneous Rifr derivative of XcmN[27]
XcmH1003Spr, Rifr derivative of XcmN[27]
Xcc049Wild-type Xcc strain, used for construction of pKMSA1/A2This lab
Xss-V2–18Wild-type, causes bacterial blight of cottonHainan University, China
M1tal3 deletion mutant of Xss-V2–18This study
M2tal2 deletion mutant of Xss-V2–18This study
M3tal2 tal4 deletion mutant of Xss-V2–18This study
M4tal2 tal4 tal5 tal6 deletion mutant of Xss-V2–18This study
M5tal2 tal3 tal4 tal5 tal6 deletion mutant of Xss-V2–18This study
M6tal-free mutant of Xss-V2–18This study
Plasmids
pBluescript II SK(−)Apr, phagemid, pUC derivativeLab collection
pMD18-TApr, pUC18 derivative, TA cloning vector, 2692 bpTaKaRa
pKMS1Kmr, sacB mob lacZ oriV, 6475 bp[60]
pHM1Broad-spectrum cosmid vector, cos, parA, IncW, Spr[61]
pKMSA1pKMS1 containing a 930-bp XbaI/ SmaI fragment; insert contains a fusion of a and b fragments that encode the N- and C-terminal sides of the tal CRR; KmrThis study
pKMSA2pKMS1 containing a 450-bp XbaI/ SmaI fragment; insert contains a fusion of the c and d fragments that encode the N- and C-terminal sides of the tal CRR; KmrThis study
pB-tal1pBluescriptIISK(−) containing tal1 of Xss-V2–18This study
pB-tal2pBluescript II SK(−) containing tal2 of Xss-V2–18This study
pB-tal3pBluescript II SK(−) containing tal3 of Xss-V2–18This study
pB-tal4pBluescript II SK(−) containing tal4 of Xss-V2–18This study
pB-tal5pBluescript II SK(−) containing tal5 of Xss-V2–18This study
pB-tal6pBluescript II SK(−) containing tal6 of Xss-V2–18This study
pZWavrXa7avrXa7 in pBluescript II KS+, contains FLAG epitope immediately downstream of the second SphI site in the C-terminus of AvrXa7, Apr[62]
pZW-tal2SphI fragment of tal2 in pZWavrXa7, AprThis study
pHZW-tal2pHM1 fused with pZW-tal2 at HindIII, lacZ promoter upstream of tal2, Apr, SprThis study