Identification of cholesterol-assimilating actinomycetes strain and application of statistical modeling approaches for improvement of cholesterol oxidase production by Streptomyces anulatus strain NEAE-94

Table 4 Three-level Box-Behnken design representing cholesterol oxidase production as influenced by agitation speed (X₁), cholesterol concentration (X₂) and yeast extract concentration (X₃)

Std	Run	Agitation speed		Cholesterol concentration		Yeast extract concentration		Cholesterol oxidase activity (U/mL)		Residuals
Std	Run	Coded	Actual (rpm)	Coded	Actual (g/L)	Coded	Actual (g/L)	Experimental	Predicted	Residuals
15	1	0	150	0	4	0	5	26.44	26.55	−0.10
13	2	0	150	0	4	0	5	25.88	26.55	−0.66
1	3	−1	100	−1	2	0	5	12.90	12.97	−0.07
9	4	0	150	−1	2	−1	3	10.31	10.50	−0.19
2	5	1	200	−1	2	0	5	11.48	10.74	0.74
7	6	−1	100	0	4	1	7	13.13	12.58	0.55
3	7	−1	100	1	6	0	5	16.29	17.03	−0.74
12	8	0	150	1	6	1	7	17.76	17.57	0.19
11	9	0	150	−1	2	1	7	8.44	8.91	−0.47
10	10	0	150	1	6	−1	3	15.03	14.56	0.47
4	11	1	200	1	6	0	5	19.47	19.40	0.07
14	12	0	150	0	4	0	5	27.31	26.55	0.77
5	13	−1	100	0	4	−1	3	5.64	5.38	0.27
6	14	1	200	0	4	−1	3	11.40	11.95	−0.55
8	15	1	200	0	4	1	7	5.89	6.16	−0.27

ISSN: 1471-2180