Strategy using a new antigenic test for rapid diagnosis of Streptococcus pneumoniae infection in respiratory samples from children consulting at hospital

Table 2 Microbiological results concerning the search for S. pneumoniae on the 196 samples of the study

Microbiological tests	Number of positive (%)
Semi-quantitative culture	70 (35.7)
- < 10⁷ CFU/ml	20 (10.2)
- ≥ 10⁷ CFU/ml	50 (25.5)
Positive PCR tests (ply and/or lytA)	169 (86.2)
- ply gene	167 (85.2)
• Ct < 22^a	63 (32.1)
• Ct ≥ 22	104 (53.1)
- lytA gene	123 (62.7)
• Ct < 23^a	32 (16.3)
• Ct ≥ 23	91 (46.4)
Positive PneumoResp RDT
- undiluted sample	133 (67.8)
- 1:100 diluted sample	76 (38.7)

Ct cycle threshold
^aThese thresholds were shown to correspond to approximately 10⁷ CFU/ml by qPCR for the considered PCR assay (see Fig. 1)

ISSN: 1471-2180