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Table 2 Microbiological results concerning the search for S. pneumoniae on the 196 samples of the study

From: Strategy using a new antigenic test for rapid diagnosis of Streptococcus pneumoniae infection in respiratory samples from children consulting at hospital

Microbiological testsNumber of positive (%)
Semi-quantitative culture70 (35.7)
 - <  107 CFU/ml20 (10.2)
 - ≥ 107 CFU/ml50 (25.5)
Positive PCR tests (ply and/or lytA)169 (86.2)
 - ply gene167 (85.2)
  • Ct < 22a63 (32.1)
  • Ct ≥ 22104 (53.1)
 - lytA gene123 (62.7)
  • Ct < 23a32 (16.3)
  • Ct ≥ 2391 (46.4)
Positive PneumoResp RDT
 - undiluted sample133 (67.8)
 - 1:100 diluted sample76 (38.7)
  1. Ct cycle threshold
  2. aThese thresholds were shown to correspond to approximately 107 CFU/ml by qPCR for the considered PCR assay (see Fig. 1)