Table 1 Specific primers of quantitative real-time PCR
From: Effects of resveratrol on cariogenic virulence properties of Streptococcus mutans
Genea | Primer sequence(5′-3′) | Description | |
|---|---|---|---|
Forward | Reverse | ||
16S rRNA | AGCGTTGTCCGGATTTATTG | CTACGCATTTCACCGCTACA | Normalizing internal standard |
ldh | GGCGACGCTCTTGATCTTAG | GGTTAGCAGCAACGAGGAAG | Lactate dehydrogenase |
relA | ACAAAAAGGGTATCGTCCGTACAT | AATCACGCTTGGTATTGCTAATTG | Guanosine tetra (penta)-phosphatesynthetase |
gtfC | GGTTTAACGTCAAAATTAGCTGTATTAGC | CTCAACCAACCGCCACTGTT | Water soluble and insoluble glucan production |
comDE | ACAATTCCTTGAGTTCCATCCAAG | TGGTCTGCTGCCTGTTGC | Competence-stimulating peptide |