Evaluation of real-time PCR targeting the lipL32 gene for diagnosis of Leptospira infection

Podgoršek, Daša; Ružić-Sabljić, Eva; Logar, Mateja; Pavlović, Andrea; Remec, Tatjana; Baklan, Zvonko; Pal, Emil; Cerar, Tjaša

doi:10.1186/s12866-020-01744-4

BMC Microbiology

Table 2 Patients (Pt) who were positive according to at least one of the three conventional PCR assays or according to RT-PCR, including results from conventional PCR product sequence analysis, and from culture and serology (i.e. microscopic agglutination test [MAT] on blood) analyses

From: Evaluation of real-time PCR targeting the lipL32 gene for diagnosis of Leptospira infection

Pt	Sample	Conventional PCR					RT-PCR	Culture	MAT
Pt	Sample	LeptoA/B	L3/4	L4/ LPato2	Final result	Product sequence analysis	RT-PCR	Culture	MAT
1	blood	pos^a	neg	pos^a	pos	^aLeptospira interrogans	pos	neg	Pos 1600 Sejroe
1	urine	pos^a	neg	pos^a	pos	^aLeptospira interrogans	pos	neg	Pos 1600 Sejroe
2	urine	pos	neg	pos	pos	NT	pos	neg	neg
3	urine	pos	neg	pos	pos	NT	pos	pos	Pos 1600 Icterohaemorrhaiae
4	urine	pos	neg	pos	pos	NT	pos	neg	Pos 600 Australis
5	blood	neg	neg	neg	neg	NT	pos	neg	Pos 25,600 Cynopteri
6	blood	pos	neg	pos	pos	NT	pos	neg	neg
7	blood	pos	neg	pos	pos	NT	pos	neg	Pos 100 Australis
8	blood	neg	neg	neg	neg	NT	pos	neg	Pos 102,400 Tarassovi
9	blood	neg	neg	neg	neg	NT	pos	neg	neg
10	urine	pos^a	neg	pos^a	pos	^aLeptospira kirschneri	neg	neg	neg
11	blood	neg	neg	neg	neg	NT	pos	neg	Pos 1600 Sejroe
12	blood	pos^a	neg	pos^a	pos	^aLeptospira spp.	pos	neg	Neg
13	urine	neg	neg	pos^a	neg	^aLeptospira spp.	pos	NT	Neg
14	blood	neg	neg	neg	neg	NT	pos	neg	Pos 800 Australis
15	blood	neg	neg	pos^a	neg	^aLeptospira interogans	pos	pos	Pos 200 Canicola
16	blood	pos^a	neg	pos^a	pos	^aLeptospira spp.	pos	neg	neg
16	urine	pos^a	neg	pos^a	pos	^aLeptospira spp.	pos	neg	neg
17	blood	neg	neg	pos^a	neg	^aLeptospira spp.	pos	pos	NT
18	blood	pos	neg	pos	pos	NT	pos	pos	Pos 400 Grippotyphosa
19	blood	pos	neg	pos	pos	NT	pos	NT	neg
20	blood	neg	neg	neg	neg	NT	pos	neg	Pos 200 Australis
20	urine	neg	neg	neg	neg	NT	pos	neg	Pos 200 Australis
21	blood	neg	neg	neg	neg	NT	pos	NT	NT
22	blood	neg	neg	pos^a	neg	^aLeptospira spp.	pos	NT	NT
23	blood	pos	neg	pos	pos	NT	pos	NT	neg
23	urine	pos	neg	pos	pos	NT	pos	NT	neg
24	urine	pos^a	neg	pos^a	pos	^aLeptospira meyeri	pos	pos	Pos 400 Sejroe
24	blood	pos^a	neg	pos^a	pos	^aLeptospira meyeri	pos	pos	Pos 400 Sejroe
25	blood	neg	neg	pos^a	neg	^aLeptospira spp.	pos	NT	Pos 200 Grippotyphosa
26	blood	neg	neg	pos^a	neg	^aLeptospira spp.	pos	neg	Pos 200 Australis
27	blood	pos^a	neg	pos^a	pos	^aLeptospira spp.	pos	neg	neg
28	urine	neg	neg	neg	neg	NT	pos	neg	neg
29	blood	neg	neg	neg	neg	NT	pos	neg	Pos 3200 Copenhageni
30	blood	neg	neg	neg	neg	NT	pos	pos	neg
31	urine	neg	neg	neg	neg	NT	pos	neg	Pos 400 Panama
32	blood	neg	neg	neg	neg	NT	pos	pos	NT
33	blood	pos^a	neg	pos^a	pos	^aLeptospira interogans	pos	pos	neg
34	blood	pos	neg	pos	pos	NT	pos	neg	neg
34	urine	pos	neg	pos	pos	NT	pos	neg	neg
35	blood	pos	neg	pos	pos	NT	pos	neg	neg
36	blood	pos^a	neg	pos^a	pos	^aLeptospira spp.	pos	neg	neg
37	urine	neg	neg	pos^a	neg	^aLeptospira spp.	pos	neg	Pos 3200 Sejroe
38	urine	pos	neg	pos	pos	NT	pos	neg	Pos 800 Grippotyphosa
39	blood	pos	neg	pos	pos	NT	pos	pos	Pos 1600 Icterohaemorrhaiae
40	urine	neg	neg	neg	neg	NT	pos	neg	Pos 200 Australis
41	blood	pos	neg	pos	pos	NT	pos	neg	neg
41	urine	neg	neg	neg	neg	NT	pos	neg	neg
42	urine	pos^a	neg	pos^a	pos	^aAtopobium vaginae	neg	neg	neg

NT not tested
^a Conventional PCR products subjected to sequence analysis

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ISSN: 1471-2180

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