Antibiotic resistance and virulence patterns of pathogenic Escherichia coli strains associated with acute gastroenteritis among children in Qatar

Table 1 Primers used to amplify selected pathogenic E. coli virulence genes

Organism	Primer sequence (5′ → 3′)	Target gene	Size (bp)	Reference
Enteropathogenic E.coli (EPEC)	GACCCGGCACAAGCATAAGC CCACCTGCAGCAACAAGAGG	eae	384	[15]
EPEC	CAGCCTTCGTTCAGATCCTA GTAGCCAGCCCCCGATGA	tir	400	[16]
EPEC	AATGGTGCTTGCGCTTCGTGC GCCGCTTTATCCAACCTGGTA	bfpA	326	[15]
Enteroaggregative E.coli (EAEC)	CTA ATT GTA CAA TCG ATG TA AGA GTC CAT CTC TTT GAT AAG	aggR	457	[17]
EAEC	CTT GGG TAT CAG CCT GAA TG AAC CCA TTC GGT TAG AGC AC	aap	310	[17]
EAEC	CTG GCG AAA GAC TGT ATC AT CAATGT ATA GAA ATC CGC TGT T	aatA	629	[17]
EAEC	ATGAATATACTATTTACGGAATC CTACAGGCACAGAAAATGCCGATG	capU	776	[16]
EAEC	CTC TTA GCA GGG AGT TTG TC GCT TTG TTT ACC GAC TGA AC	aaiA	430	[18]
EAEC	CCA TCA ACA CAG TAT ATC CGA GGT CGC GAG TGA CGG CTT TGT	astA	111	[18]
EAEC	CACAGTATATCCGAAGGC CGAGTGACGGCTTTGTAG	east	97	[19]
* EPEC/EHEC	ATAAATCGCCTATCGTTGACTAC AGAACGCCCACTGAGATCATC	SXT1	180	[15]
* EPEC/EHEC	GGCACTGTCTGAAACTGCTCC TCGCCAGTTATCTGACATTCTG	SXT2	225	[15]

*The Enterohemorrhagic E. coli (EHEC) pathotype can be identified by the presence of the eae gene along with stx1 gene, stx2 gene, or both [20]. Therefore, to differentiate between EHEC and EPEC we screen for the presence of stx1 gene, stx2 gene

ISSN: 1471-2180