Fig. 6

Comparison of BAS3321 rhAmp assay regions in Sterne and BcbvaCI. Forward and reverse primers developed for rhAmp assays are depicted in yellow and grey, respectively, with location of anthrose SNPs shown in blue. Two SNPs are located at the 5′ end of 892 forward primer and at position 933 of the 892 reverse primer (red). The anthrose operon and BAS3321 gene of Sterne are remarkedly conserved in Bcvba, exhibiting 99.1 and 99% identity, respectively with their homologs