Fig. 2
The effect of Ab on human respiratory epithelial cells in different incubation states. a Microscopy observation of the effect of Ab on epithelial cells (4 × 105 cells per well) in different incubation states. Scale bar = 30 μm. Experimental group: addition of bacterial suspension; Control group: addition of 0.45% NaCl; Red arrows: bubble-like (denucleated) dead cells; Yellow arrows: the phenomenon of bacterial aggregation; Blue arrows: massive cell death. b Ab infection TCRPs of epithelial cells (20,000 cells per well) in different incubation states. I: addition of bacterial suspension (red arrow) at cell suspension (0 h); II: addition of bacterial suspension (green arrow) at 30–40% cell confluence (after 24 h); III: addition of bacterial suspension (blue arrow) at 80–90% cell confluence (after 48 h); IV: blank control (without intervention); t: treatment time point; t1: cell suspension (0 h); t2: 30–40% cell confluence (after 24 h); t3: 80–90% cell confluence (after 48 h). Ab infection caused the CI to rise and fall. A unique “double peak” phenomenon emerged during Ab infection. The addition of 0.45% NaCl or sterile distilled water had no significant effect on the CI