Persistent and sporadic Listeria monocytogenes strains do not differ when growing at 37 °C, in planktonic state, under different food associated stresses or energy sources

Table 1 Description of treatments for the extrinsic stress and energy source test, including pre-growth, treatment media formulation, and replicates tested

Treatments	Replicates^a	Pre-growth media	Energy Source	Salt (%)	pH levels	BAC^b (μg/mL)
Stress Conditions (tested in nutritive media [BHI])
Control	3	BHI	Dextrose	0.5	7.20	0
5% NaCl	4	BHI	Dextrose	5	7.20	0
10% NaCl	4	BHI	Dextrose	10	7.20	0
pH of 5.2	3	BHI	Dextrose	0.5	5.20	0
pH of 9.2	3	BHI	Dextrose	0.5	9.20	0
BAC 2 μg/mL	6	BHI	Dextrose	0.5	7.20	2
BAC 5 μg/mL	6	BHI	Dextrose	0.5	7.20	5
Energy Source Utilization (tested in chemically defined media [DM])
DM Control	3	DM Glucose	Glucose	0.5	6.75	0
DM Cellobiose	6	DM Glucose	Cellobiose	0.5	6.75	0
DM Fructose	4	DM Glucose	Fructose	0.5	6.75	0
DM Glycogen	6	DM Glucose	Glycogen	0.5	6.75	0
DM Lactose	4	DM Glucose	Lactose	0.5	6.75	0
DM Sucrose	6	DM Glucose	Sucrose	0.5	6.75	0

^a Number of resuscitated, biologically independent replicates that were used for the corresponding treatment
^b BAC, Benzalkonium Chloride

ISSN: 1471-2180