Fig. 2

Performance comparison between the ICW assay and standard CFU count upon S. flexneri infection. a HeLa cell monolayers were infected with the M90 T strain at MOI 100. Non-infected control cells (CC) were used as control. At the selected time points (hours post-infection, HPI), cells were either lyzed for CFU/ml count or immunostained for the ICW assay. Representative images of wells used for the ICW assay from three independent experiments performed in quadruplicate are shown. b Histogram shows the mean values ± SD of CFU/ml count (blue bars) and a.u. values (red bars). For both methods, equations and R² values of trendlines are shown. c The linear correlation (dotted black line) with relative equation and the coefficient of determination (R² value) are shown