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Table 2 Identification of Vibrio spp. based on culture method, Gram stain and biochemical tests

From: Prevalence, antimicrobial susceptibility and plasmid profiling of Vibrio spp. isolated from cultured groupers in Peninsular Malaysia

SpeciesNo. of isolatesTCBSGram stainLDCONPGOxidaseCatalaseTSI
V. communis106YellowGram negative+++A/A, no gas, no H2S
V. parahemolyticus95GreenGram negative+++A/A, no gas, no H2S; K/A, no gas, no H2S
V. alginolyticus70YellowGram negative+++A/A, no gas, no H2S
V. vulnificus52GreenGram negative+++A/A, no gas, no H2S
V. rotiferianus11GreenGram negative++A/A, no gas, no H2S
V. campbellii9GreenGram negative++A/A, no gas, no H2S
V. mytili8GreenGram negative++A/A, no gas, no H2S
V. furnissii7YellowGram negative++A/A, no gas, no H2S
V. harveyi5YellowGram negative+++A/A, no gas, no H2S
V. tubiashii4YellowGram negative++A/A, no gas, no H2S
V. fluvialis1GreenGram negative++A/A, no gas, no H2S
V. diabolicus1YellowGram negative++A/A, no gas, no H2S
Vibrio sp.11YellowGram negative++A/A, no gas, no H2S
  1. TCBS thio-sulphate citrate bile salt sucrose, LDC lysine decarboxylase, ONPG O-nitrophenyl-beta-D-galactosifase, TSI triple sugar iron, +: Positive, −: Negative, A/A Acidic slant/Acidic butt, K/A Alkaline slant/Acidic butt