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Table 2 Identification of Vibrio spp. based on culture method, Gram stain and biochemical tests

From: Prevalence, antimicrobial susceptibility and plasmid profiling of Vibrio spp. isolated from cultured groupers in Peninsular Malaysia

Species

No. of isolates

TCBS

Gram stain

LDC

ONPG

Oxidase

Catalase

TSI

V. communis

106

Yellow

Gram negative

+

–

+

+

A/A, no gas, no H2S

V. parahemolyticus

95

Green

Gram negative

+

–

+

+

A/A, no gas, no H2S; K/A, no gas, no H2S

V. alginolyticus

70

Yellow

Gram negative

+

–

+

+

A/A, no gas, no H2S

V. vulnificus

52

Green

Gram negative

+

–

+

+

A/A, no gas, no H2S

V. rotiferianus

11

Green

Gram negative

–

–

+

+

A/A, no gas, no H2S

V. campbellii

9

Green

Gram negative

–

–

+

+

A/A, no gas, no H2S

V. mytili

8

Green

Gram negative

–

–

+

+

A/A, no gas, no H2S

V. furnissii

7

Yellow

Gram negative

–

–

+

+

A/A, no gas, no H2S

V. harveyi

5

Yellow

Gram negative

+

–

+

+

A/A, no gas, no H2S

V. tubiashii

4

Yellow

Gram negative

–

–

+

+

A/A, no gas, no H2S

V. fluvialis

1

Green

Gram negative

–

–

+

+

A/A, no gas, no H2S

V. diabolicus

1

Yellow

Gram negative

–

–

+

+

A/A, no gas, no H2S

Vibrio sp.

11

Yellow

Gram negative

–

–

+

+

A/A, no gas, no H2S

  1. TCBS thio-sulphate citrate bile salt sucrose, LDC lysine decarboxylase, ONPG O-nitrophenyl-beta-D-galactosifase, TSI triple sugar iron, +: Positive, −: Negative, A/A Acidic slant/Acidic butt, K/A Alkaline slant/Acidic butt