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Fig. 4 | BMC Microbiology

Fig. 4

From: Identification of specific B cell linear epitopes of mycoplasma hyorhinis P37 protein using monoclonal antibodies against baculovirus-expressed P37 protein

Fig. 4

Western blot analysis of four mAbs to Mhr. a Western blot analysis revealed four mAbs (C6, C8, E1, and E6). A specific Mhr band was detected at 43.3 kDa, indicating that the P37 protein expressed by the eukaryotic expression system had good immunogenicity. Lane M, PageRuler™ Prestained Protein Ladder; lane Mhr, Mhr lysate. b Immunohistochemical analysis of Mhr-specific antigens in infected lung tissue using prepared mAbs. Positive signals (red arrows) were detected for the Mhr-specific antigen in infected lung tissue (P-Mhr) but were not detected in Mhp positive lung tissue (P-Mhp) and uninfected lung tissue (N)

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