Table 1 Primers used in this study
Primer | Sequence (5′ → 3′) | Note |
|---|---|---|
Po-gpdF | GGTACCTTTATTGGCGGT | Promoter cloning |
Po-gpdR | CCAGGTCAGTGAAATTTCC | Â |
pal1_gF | ATGACAATCCTATCCGCAGAC | gDNA fragment cloning |
pal1_gR | TTAGAATAAATCAACGATGATA | Â |
pal1_cF | ATGACAATCCTATCCGCAGAC | cDNA fragment cloning |
pal1_cR | TTAGAATAAATCAACGATGATA | Â |
pal2_gF | ATGACTATTCTCTCAGGGA | gDNA fragment cloning |
pal2_gR | CTACGCGAACATCGCTA | Â |
pal2_cF | ATGACTATTCTCTCAGGGA | cDNA fragment cloning |
pal2_cR | CTACGCGAACATCGCTA | Â |
pal1-qF | CTCCTTCACAATCGCATCTA | qPCR |
pal1-qR | CTTCAGCCGCCTATGTTG | Â |
pal2-qF | CAACTGCTGCGTATGTCA | Â |
pal2-qR | GATGTAGAGGTATGAGGAGATT | Â |
β-actin-F | GCGATGAACAATAGCAGGG | Endogenous control |
β-actin-R | GCTGGTATCCACGAGACAAC |  |
pal1-OE-F | ttacaggtcaaagttATGACAATCCTATCCGCAGAC | Construction of OE plasmids |
pal1-OE-R | aattctagagggcccTTAGAATAAATCAACGATGATA | Â |
pal2-OE-F | ttacaggtcaaagttATGACTATTCTCTCAGGGA | Â |
pal2-OE-R | aattctagagggcccCTACGCGAACATCGCTA | Â |
pal1-RNAi-F1 | actgacctggGATTTGCAACCGTTGTCTTACG | Construction of RNAi plasmids |
pal1-RNAi-R1 | gttggagtgcaactccaCTAAAATCAGATGAGGTTGTAAGCG | Â |
pal1-RNAi-F2 | tagTGGAGTTGCACTCCAACGTGA | Â |
pal1-RNAi-R2 | catgccaattctagagggcccGATTTGCAACCGTTGTCTTACG | Â |
pal2-RNAi-F1 | aatttcactgacctggCCACCGACAATCCTCTCATCG | Â |
pal2-RNAi-R1 | gcacaaccaagcagtaaaCTAGAAAATGAGAATAAGACCTTGCTACC | Â |
pal2-RNAi-F2 | agTTTACTGCTTGGTTGTGCATTTC | Â |
pal2-RNAi-R2 | catgccaattctagagggcccCCACCGACAATCCTCTCATCG | Â |
hyg F | CGACAGATCCGGTCGGCATCTACTCTATTTCTT | Detection of transformants |
hyg R | TCTCGTGCTTTCAGCTTCGATGTAGGAGGG | Â |
OE_gpd_pal1F | TGCGTGGTAGAAGAATGG | Â |
OE_gpd_pal1R | CGATGAAGAAGGTAGAATGC | Â |
OE_gpd_pal2F | CGTTCTCCGAGTCTGTTC | Â |
OE_gpd_pal2R | TGATAGCGTCTTGCCATC | Â |
pal1-PE-F | TCGCGGATCCGAATTCATGCAATCCTATCCGCA GAC | Construction of prokaryotic expression plasmid |
pal1-PE-R | GAGTGCGGCCGCTTAGAATAAATCAACGATATAGGC | Â |
pal2-PE-F | CGGATCCGAATTCAGACTATTCTCTAGGGACCACCG | Â |
pal2-PE-R | TGCTCGAGTGCGGCCGCCTACGCAACATCGCTACCG | Â |