Fig. 5

Comparison of DNA yields from clinical skin and wound swabs using kit-based extraction (PS1) and the method described here (PS2). DNA was quantified fluorometrically by Qubit assay. Limit of detection is indicated by the dashed line (LOD for the Qubit dsDNA HS assay = 10 pg/μL according to supplier documentation [21]). The fraction of samples above the LOD (n) is listed. All negative controls, which were exposed to air in the collection room or blank extractions, were below the LOD