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Fig. 3 | BMC Microbiology

Fig. 3

From: Improved single-swab sample preparation for recovering bacterial and phage DNA from human skin and wound microbiomes

Fig. 3

Quantitative recovery of M13 loaded onto skin and then swabbed immediately (blue) or after drying (orange), as determined by qPCR. Expected or observed DNA copies per swab is indicated. Complete recovery is indicated by the dotted green line. A negative control (red) indicates experimental background determined by loading of 1X TE onto skin followed by swabbing and qPCR, and therefore reflects the combined background from skin, swabbing and extraction, and qPCR reagents. Error bars indicate standard deviation among biological triplicates

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