Toxigenic potential and antimicrobial susceptibility of Bacillus cereus group bacteria isolated from Tunisian foodstuffs

Table 1 Primers used in the simplex PCR for the detection of virulence genes in B. cereus

Targeted Gene	Primer name	Sequence (5′- 3′)	Product Size (bp)	Annealing Temp (°C)	Reference
hblA	HA F HA R	AAGCAATGGAATACAATGGG AGAATCTAAATCATGCCACTGC	1154	56	[33]
hblB	HA F HB R	AAGCAATGGAATACAATGGG AATATGTCCCAGTACACCCG	2684	58	[33]
hblC	HC F HC R	GATACTCAATGTGGCAACTGC TTGAGACTGCTCGTCTAGTTG	740	58	[33]
hblD	HD F HD R	ACCGGTAACACTATTCATGC GAGTCCATATGCTTAGATGC	829	58	[33]
nheA	NA F NA R	GTTAGGATCACAATCACCGC ACGAATGTAATTTGAGTCGC	755	56	[33]
nheB	NB F NB R	TTTAGTAGTGGATCTGTACGC TTAATGTTCGTTAATCCTGC	743	54	[33]
nheC	NC F NC R	TGGATTCCAAGATGTAACG ATTACGACTTCTGCTTGTGC	683	54	[33]
bceT	bceT-f bceT-r	GCTACGCAAAAACCGAGTGGTG AATGCTCCGGACTATGCTGACG	679	57	[12]
cytK	CK F CK R	ACAGATATCGG(G,T)CAAAATGC TCCAACCCAGTT(A,T)(G,C) CAGTTC	809	54	[11]
cytK-1	CK1 F CK1 R	CAATTCCAGGGGCAAGTGTC CCTCGTGCATCTGTTTCATGAG	426	57	[11]
cytK-2	CK2 F CK2 R	CAATCCCTGGCGCTAGTGCA GTGIAGCCTGGACGAAGTTGG	585	57	[11]
ces	EM1F EM1R	GACAAGAGAAATTTCTACGAGCAAGTAAT GCAGCCTTCCAATTACTCCTTCTGCCACAGT	635	58	[34]
ces	CesF1 CesR2	GGTGACACATTATCATATAAGGTG GTAAGCGAACCTGTCTGTAACAACA	1271	58	[35]

ISSN: 1471-2180