Fig. 2
Sensitivity analysis of the real-time PCR assay. Different concentrations of V. parahaemolyticus DNA template (1.0 × 106 to 1.0 × 100 copies/reaction) were amplified by either real-time RPA or real-time PCR. As shown in this figure, the detection limit for both was 1.0 × 102 copies/reaction. RPA assay is shown in panel A and real-time PCR is shown in panel B. The concentrations used as a template for reactions 1–7 were 1.0 × 106, 1.0 × 105, 1.0 × 104, 1.0 × 103, 1.0 × 102, 1.0 × 101 and 1.0 × 100 copies/reaction. Shown in this figure is one representative plot out of five independent reactions for real-time RPA